PMID- 7930832
OWN - NLM
STAT- MEDLINE
DCOM- 19941103
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 66
IP  - 3
DP  - 1994 Sep
TI  - Use of fluorescence in situ hybridization to measure chromosome aberrations as a 
      predictor of radiosensitivity in human tumour cells.
PG  - 297-307
AB  - Fluorescence in situ hybridization (FISH) is a potential assay for determining 
      cellular radiosensitivity based on the detection of chromosome damage. This 
      approach was chosen because of its relative simplicity and short assay time. Two 
      radiosensitive and two radioresistant human tumour cell lines were used. The 
      radiosensitive lines were an ovarian carcinoma line (A1847) and a squamous 
      carcinoma line (SCC61). The radioresistant cells were a lung adenocarcinoma line 
      (A549) and a second squamous line (SQ20B). Whole chromosome-specific probes were 
      used to detect radiation-induced chromosome aberrations in mitotic cells. 
      Available probes were first screened to characterize the intrinsic chromosome 
      aberrations before irradiation and the appropriate probes (minimum fluorescent 
      spots) were selected for each cell line. Maximum radiation-induced aberrations 
      were found 24 h after irradiation. Dose-response curves corrected for target size 
      (proportion of genome probed) differed for all cell lines. The radiosensitive 
      A1847 cell line showed more induced aberrations compared with the radioresistant 
      A549 cell line, in agreement with the survival data. In contrast, the SQ20B cell 
      line showed more induced chromosome aberrations than the more radiosensitive 
      SCC61 cell line, leading to the hypothesis that the SQ20B cells could tolerate 
      more aberrations. Dose-response curves obtained in surviving cells 14 days 
      postirradiation indeed showed elevated levels of chromosome aberrations for SQ20B 
      cells. The difference in chromosome aberrations between 1 and 14 days showed a 
      good correlation with the survival data for all four cell lines. In conclusion, 
      FISH of mitotic cells with whole chromosome probes appears to be a suitable assay 
      to predict radiosensitivity. It seems necessary, however, to determine both 
      induced and remaining chromosome aberrations, since different processing or 
      tolerance of radiation-induced aberrations, including stable types, could lead to 
      different correlations with cell survival.
FAU - Coco-Martin, J M
AU  - Coco-Martin JM
AD  - Division of Experimental Therapy, The Netherlands Cancer Institute Plesmanlaan 
      121, Amsterdam.
FAU - Smeets, M F
AU  - Smeets MF
FAU - Poggensee, M
AU  - Poggensee M
FAU - Mooren, E
AU  - Mooren E
FAU - Hofland, I
AU  - Hofland I
FAU - van den Brug, M
AU  - van den Brug M
FAU - Ottenheim, C
AU  - Ottenheim C
FAU - Bartelink, H
AU  - Bartelink H
FAU - Begg, A C
AU  - Begg AC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
SB  - IM
MH  - Cell Survival/radiation effects
MH  - *Chromosome Aberrations
MH  - Dose-Response Relationship, Radiation
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Radiation Tolerance
MH  - Tumor Cells, Cultured
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - X10KCWL0JEPUG58W [pii]
AID - 10.1080/09553009414551231 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1994 Sep;66(3):297-307. doi: 10.1080/09553009414551231.