PMID- 7930942
OWN - NLM
STAT- MEDLINE
DCOM- 19941102
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 56
IP  - 4
DP  - 1994 Oct
TI  - Influence of acridine tracer dyes on neutrophil function.
PG  - 464-8
AB  - A study was performed to elucidate the effect of two commonly used fluorescent 
      dyes in in vivo microscopic studies, acridine orange (AO) and acridine red (AR), 
      on the ability of phorbol myristate acetate (PMA)- or formyl peptide 
      (fMLP)-stimulated human neutrophils to adhere to a bovine serum albumin matrix 
      and to generate superoxide anions (SOX). Unlabeled stimulated human neutrophils 
      showed 36 +/- 9% (PMA, 10(-7) M) and 11 +/- 7% (fMLP, 10(-7) M) adherence to the 
      matrix. This adhesion was CD18 dependent as evidence by 98% and 92% reduction, 
      respectively, when the anti-CD18 antibody IB4 was included. A dose-dependent 
      inhibition of stimulated human neutrophil adhesion was evident after 30 min of in 
      vitro dye labeling and the EC50 was approximately 70 micrograms/ml (AR) and 145 
      micrograms/ml (AO). SOX generation by PMA-stimulated neutrophils was unaffected 
      up to 100 micrograms/ml AR and AO but was reduced by 40-60% at higher doses. 
      Rabbit neutrophils labeled in vivo or in vitro with 100 micrograms/ml AR 
      exhibited 41% and 61% lower SOX generation, respectively. The study indicate that 
      neutrophil function, in terms of ability to adhere to a BSA matrix using 
      CD11/CD18 integrins and to generate SOX upon stimulation, is reduced depending on 
      the dose and choice of fluorescent dye. Caution should be exercised when using 
      these compounds at high concentrations in studies of PMN function.
FAU - Hansell, P
AU  - Hansell P
AD  - La Jolla Institute for Experimental Medicine, California.
FAU - Berger, E
AU  - Berger E
FAU - Chambers, J D
AU  - Chambers JD
FAU - Arfors, K E
AU  - Arfors KE
LA  - eng
GR  - HL-46022/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Rhodamines)
RN  - 0KGA4B4N1H (acridine red)
RN  - 11062-77-4 (Superoxides)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Neutrophils/*drug effects
MH  - Rabbits
MH  - Respiratory Burst/drug effects
MH  - Rhodamines/*pharmacology
MH  - Superoxides/metabolism
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1002/jlb.56.4.464 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1994 Oct;56(4):464-8. doi: 10.1002/jlb.56.4.464.