PMID- 7931844
OWN - NLM
STAT- MEDLINE
DCOM- 19941107
LR  - 20111117
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 173
IP  - 3
DP  - 1994 Jul
TI  - Detection of specimen contamination in routine histopathology by HLA class II 
      typing using the polymerase chain reaction and sequence specific oligonucleotide 
      probing.
PG  - 243-8
AB  - This study evaluates the use of human leukocyte antigen (HLA) class II PCR-SSO 
      typing for the investigation of suspected specimen contamination in four routine 
      surgical histopathology cases. Two cases were of patients undergoing 
      transurethral resection of the prostate gland. The third case was a patient 
      undergoing excision of a subcutaneous small cell carcinoma. The fourth case was a 
      patient undergoing reversal of a Hartman's procedure for adenocarcinoma of the 
      colon. Tissue was extracted from routinely processed formalin-fixed, 
      paraffin-embedded material and HLA class II typed, using a non-radioactive 
      polymerase chain reaction-sequence specific oligonucleotide probe technique 
      (PCR-SSO), using probes specific for the DRB, DQA, and DQB loci. The accuracy of 
      PCR-SSO typing of DNA extracted from paraffin biopsy material was confirmed by 
      concordant results for DRB, DQA, and DQB typing in five separate control 
      individuals from whom frozen and paraffin lymph node biopsies were available. 
      PCR-SSO typing was also successful in the four study cases and revealed that 
      contamination had occurred in two cases, eliminating this possibility in the 
      remaining cases. This study demonstrates that DNA can be reliably amplified and 
      accurately typed from routinely processed material, and reveals a useful new 
      application for PCR-SSO tissue typing.
FAU - Bateman, A C
AU  - Bateman AC
AD  - Department of Histopathology, Southampton General Hospital, U.K.
FAU - Leung, S T
AU  - Leung ST
FAU - Howell, W M
AU  - Howell WM
FAU - Roche, W R
AU  - Roche WR
FAU - Jones, D B
AU  - Jones DB
FAU - Theaker, J M
AU  - Theaker JM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 0 (HLA-DQbeta antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Adenocarcinoma/immunology
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Small Cell/immunology
MH  - Colonic Neoplasms/immunology
MH  - Diagnostic Errors
MH  - HLA-DQ Antigens/analysis
MH  - HLA-DQ alpha-Chains
MH  - HLA-DQ beta-Chains
MH  - HLA-DR Antigens/analysis
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Lymph Nodes/immunology
MH  - Male
MH  - *Oligonucleotide Probes
MH  - Paraffin Embedding
MH  - *Polymerase Chain Reaction
MH  - Prostate/immunology
MH  - Skin Neoplasms/immunology
MH  - *Specimen Handling
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1002/path.1711730307 [doi]
PST - ppublish
SO  - J Pathol. 1994 Jul;173(3):243-8. doi: 10.1002/path.1711730307.