PMID- 7933630
OWN - NLM
STAT- MEDLINE
DCOM- 19941122
LR  - 20170214
IS  - 1011-8942 (Print)
IS  - 1011-8942 (Linking)
VI  - 8
IP  - 1
DP  - 1994 Jun
TI  - Increased glycosylation of human lens epithelial basement membrane in diabetes 
      mellitus.
PG  - 26-31
AB  - We studied the nonenzymatic glycosylation of lens epithelial basement membranes 
      (LEBM) of senile cataractous lenses of both diabetic and nondiabetic patients. 
      The human LEBMs were isolated from surgically removed senile cataracts and 
      purified by osmotic lysis and detergent treatments. Glycosylation assay of LEBMs 
      was done using the colorimetric method of Fluckiger and Winterhalter. The 
      glycosylation value ranged from 16.39 to 92.56 n mol/mg protein overall, with a 
      mean of 63.54 +/- 24.56 n mol/mg protein for the diabetic specimens and a mean of 
      29.97 +/- 14.48 n mol/mg protein for the nondiabetic controls (P = 0.009). The 
      study confirms our previous observation of in vivo glycosylation of the LEBM and 
      further establishes that diabetic patients have a twofold increase in the amount 
      of LEBM glycosylation when compared to their nondiabetic counterparts.
FAU - Shin, D H
AU  - Shin DH
AD  - Kresge Eye Institute, Wayne State University School of Medicine, Detroit, 
      Michigan 48201.
FAU - Mandel, S S
AU  - Mandel SS
FAU - Lee, J H
AU  - Lee JH
FAU - Ernst, B
AU  - Ernst B
FAU - Newman, B L
AU  - Newman BL
FAU - Juzych, M
AU  - Juzych M
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Korean J Ophthalmol
JT  - Korean journal of ophthalmology : KJO
JID - 8804513
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Aged
MH  - Basement Membrane/metabolism
MH  - Cataract/metabolism
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Epithelium/metabolism
MH  - Female
MH  - Glucose/metabolism
MH  - Glycosylation
MH  - Humans
MH  - Lens, Crystalline/*metabolism
MH  - Male
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.3341/kjo.1994.8.1.26 [doi]
PST - ppublish
SO  - Korean J Ophthalmol. 1994 Jun;8(1):26-31. doi: 10.3341/kjo.1994.8.1.26.