PMID- 7934162
OWN - NLM
STAT- MEDLINE
DCOM- 19941104
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 8
IP  - 10
DP  - 1994 Oct
TI  - Molecular cloning and analysis of the human Tec protein-tyrosine kinase.
PG  - 1663-72
AB  - Mouse Tec is a non-receptor type protein-tyrosine kinase and is highly expressed 
      in many hematopoietic cell lines. To investigate the roles of the Tec kinase in 
      the human hematopoietic system, we isolated cDNAs encoding the human Tec kinase. 
      The human tec cDNAs can encode a peptide of 631 amino acid residues with a 
      calculated molecular mass of 73,624. The predicted human Tec protein is highly 
      homologous to those of the members of the Tec family including mouse Tec type IV 
      (94% homology), mouse Tsk/Itk (60%), and human Btk (57%). The homology between 
      human Tec and other members of the Tec family can be observed not only in the Src 
      homology 3 (SH3), SH2, and kinase domains, but also in the N-terminal unique 
      domain. Northern blot analysis demonstrated that the major transcripts of tec 
      could be detected at 2.6 kb and 3.6 kb in a wide range of human hematopoietic 
      cell lines including myeloid, B-, and T-cell lineages. Interestingly, high 
      expression of the tec gene could be detected in all of the three patients 
      examined with myelodysplastic syndrome. The human tec gene was mapped by 
      fluorescence in situ hybridization (FISH) to chromosome 4p12.
FAU - Sato, K
AU  - Sato K
AD  - Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 
      Japan.
FAU - Mano, H
AU  - Mano H
FAU - Ariyama, T
AU  - Ariyama T
FAU - Inazawa, J
AU  - Inazawa J
FAU - Yazaki, Y
AU  - Yazaki Y
FAU - Hirai, H
AU  - Hirai H
LA  - eng
SI  - GENBANK/D29767
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 4
MH  - Cloning, Molecular
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Myelodysplastic Syndromes/genetics
MH  - Protein-Tyrosine Kinases/chemistry/*genetics
MH  - Sequence Homology, Nucleic Acid
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1994 Oct;8(10):1663-72.