PMID- 7942299
OWN - NLM
STAT- MEDLINE
DCOM- 19941108
LR  - 20190622
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 351
DP  - 1993
TI  - Secretion of monocyte chemoattractant protein-1 (MCP-1) by human mononuclear 
      phagocytes.
PG  - 55-64
AB  - Concentrations of MCP-1 and NAP-1 in culture fluids of human leukocytes were 
      measured by sandwich ELISA. PPD caused PBMC's from tuberculin-sensitive subjects 
      to secrete MCP-1 and NAP-1. PPD did not stimulate secretion by cells from a 
      tuberculin-negative subject. Since the amounts secreted were more than could be 
      produced by the few PPD-sensitized lymphocytes in the culture, we postulate that 
      other cells were stimulated to secrete these chemoattractants. This study 
      evaluated secretory capacity of one of the cell types in the PBMC culture. 
      Unstimulated monocytes did not secrete MCP-1 or NAP-1. In order of increasing 
      effect, IL-2 + IFN gamma, IL-1 alpha, and LPS caused monocyte secretion of MCP-1. 
      The rank order for NAP-1 secretion was the same. TNF alpha did not cause 
      secretion of MCP-1, but caused about the same amount of NAP-1 secretion as IL-2 + 
      IFN gamma. Composition of the culture medium was especially critical for 
      LPS-induced secretion of MCP-1, which was greatly enhanced by FCS and by Iscove's 
      DMEM compared to RPMI 1640. IL-4 inhibited LPS-induced secretion of both MCP-1 
      and NAP-1. Secretory patterns were also a function of mononuclear phagocyte 
      phenotype. LPS-induced secretion of MCP-1 was much greater for monocytes cultured 
      several days in CSF-1 than for freshly isolated monocytes. LPS stimulation of 
      bronchoalveolar macrophages caused NAP-1 secretion, but no secretion of MCP-1 
      above a relatively low baseline level.
FAU - Leonard, E J
AU  - Leonard EJ
AD  - Immunopathology Section, National Cancer Institute, Frederick, Maryland.
FAU - Skeel, A
AU  - Skeel A
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Rankin, J
AU  - Rankin J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tuberculin)
SB  - IM
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*metabolism/pharmacology
MH  - Cytokines/pharmacology
MH  - Humans
MH  - Interleukin-8/metabolism/pharmacology
MH  - Leukocytes, Mononuclear/immunology/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/physiology
MH  - Monocytes/physiology
MH  - Phagocytes/*physiology
MH  - Tuberculin/immunology
RF  - 14
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/978-1-4615-2952-1_7 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 1993;351:55-64. doi: 10.1007/978-1-4615-2952-1_7.