PMID- 7943176
OWN - NLM
STAT- MEDLINE
DCOM- 19941107
LR  - 20201226
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 145
IP  - 4
DP  - 1994 Oct
TI  - Tumor-derived monocyte chemoattractant protein-1 induces intratumoral 
      infiltration of monocyte-derived macrophage subpopulation in transplanted rat 
      tumors.
PG  - 856-67
AB  - By immunohistochemistry using anti-rat macrophage monoclonal antibodies RM-1, 
      ED1, ED2, ED3, TRPM-3, and Ki-M2R, we studied transplanted rat tumors of 9L (rat 
      gliosarcoma), Ad-2 (rat mammary carcinoma), and MT-P (rat malignant fibrous 
      histiocytoma) cell lines to examine the distribution pattern of macrophages 
      within and around the tumors. Most tumor-associated macrophages expressed RM-1, 
      ED1, and Ia antigens, indicating activated macrophages. Based on differences in 
      their immunophenotypical expression, these macrophages were distinguished into 
      two major subpopulations. One expressed TRPM-3 and/or ED3, and the other was 
      positive for ED2 and Ki-M2R. The former was considered to be monocyte-derived 
      macrophages, whereas the latter showed the immunophenotype of tissue-fixed, 
      resident macrophages. Infiltration and distribution patterns in the two 
      macrophage subpopulations differed in the three different tumors. 
      Monocyte-derived, activated macrophages infiltrated into 9L- and 
      Ad-2-transplanted tumors, which markedly produced monocyte chemoattractant 
      protein-1 (MCP-1). Additionally, numerous ED2- and Ki-M2R-positive macrophages 
      were observed within the Ad-2-transplanted tumors, and some of them expressed 
      TRPM-3. However, there were few macrophages in the MT-P-transplanted tumors that 
      showed no MCP-1 production. In transplanted tumors of four MT-P/MCP-1 cell lines 
      established by transfecting a rat MCP-1 gene expression vector (pCEP4/MCP-1) into 
      the MT-P cell line, different levels of MCP-1 production were detected, which 
      correlated well with the numbers of intratumorally infiltrated TRPM-3-positive 
      macrophages. In contrast, ED2- and Ki-M2R-positive macrophages were not detected 
      in any MT-P/MCP-1-transplanted tumors. MT-P/MCP-1-transplanted tumors exhibited 
      lower growth rate than parental MT-P-transplanted tumors. These results indicate 
      that tumor-derived MCP-1 induces intratumoral infiltration of monocyte-derived 
      macrophages, but not macrophages with the immunophenotype of tissue-fixed, 
      resident type. The former population of macrophages seems to have a suppressive 
      effect on the growth of tumors.
FAU - Yamashiro, S
AU  - Yamashiro S
AD  - Second Department of Pathology, Kumamoto University School of Medicine, Japan.
FAU - Takeya, M
AU  - Takeya M
FAU - Nishi, T
AU  - Nishi T
FAU - Kuratsu, J
AU  - Kuratsu J
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Ushio, Y
AU  - Ushio Y
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
SB  - IM
MH  - Animals
MH  - Cell Line, Transformed
MH  - Chemokine CCL2
MH  - Chemotactic Factors/genetics/*pharmacology
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Macrophages/classification/*physiology
MH  - Male
MH  - Monocytes/cytology
MH  - *Neoplasm Transplantation
MH  - Neoplasms, Experimental/*pathology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Inbred Strains
MH  - Transfection
PMC - PMC1887319
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
PMCR- 1995/04/01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PHST- 1995/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1994 Oct;145(4):856-67.