PMID- 7943274
OWN - NLM
STAT- MEDLINE
DCOM- 19941122
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 4 Pt 1
DP  - 1994 Oct
TI  - MCP-1-stimulated monocyte attachment to laminin is mediated by beta 2-integrins.
PG  - C1112-8
AB  - Migration of monocytes to sites of inflammation involves a series of attachments 
      and detachments to extracellular matrix proteins. We examined the capacity of a 
      chemokine, monocyte chemoattractant protein-1 (MCP-1), to regulate attachment of 
      human monocytes to laminin, collagen I, collagen IV, or fibronectin. MCP-1 
      increased monocyte attachment to laminin in a dose- and time-dependent manner and 
      stimulated a lesser increase to the other matrix proteins. Function-blocking 
      monoclonal antibodies (MAbs) to the integrin beta 2-subunit (CD18), including 
      Fab' fragments and alpha M (CD11b) blocked > 70% of attachment, whereas MAbs to 
      the beta 1-integrin subunit reduced attachment by < 30%. This suggests that the 
      CD11b/CD18 integrin is the predominant molecule involved in adhesion of 
      MCP-1-stimulated monocytes to laminin. The association of CD11b with F-actin 
      illustrated by confocal microscopy further supports this concept. In contrast, 
      when monocytes were stimulated with the beta 1-stimulatory MAb TS2/16, monocyte 
      adhesion to laminin occurred through beta 1-integrins. Thus MCP-1 can stimulate 
      monocyte attachment to laminin, and this process is mediated through beta 
      2-integrins, principally CD11b/CD18.
FAU - Jiang, Y
AU  - Jiang Y
AD  - Department of Oral Biology, Boston University School of Graduate Dentistry, 
      Boston University School of Medicine 02118.
FAU - Zhu, J F
AU  - Zhu JF
FAU - Luscinskas, F W
AU  - Luscinskas FW
FAU - Graves, D T
AU  - Graves DT
LA  - eng
GR  - DE-07559/DE/NIDCR NIH HHS/United States
GR  - DE-09581/DE/NIDCR NIH HHS/United States
GR  - HL-36028/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Integrins)
RN  - 0 (Laminin)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Cell Adhesion
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*pharmacology
MH  - Cytokines/pharmacology
MH  - Extracellular Matrix Proteins
MH  - Humans
MH  - Integrins/classification/*physiology
MH  - *Laminin
MH  - Microscopy, Confocal
MH  - Monocytes/*physiology
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1152/ajpcell.1994.267.4.C1112 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Oct;267(4 Pt 1):C1112-8. doi: 
      10.1152/ajpcell.1994.267.4.C1112.