PMID- 7943349
OWN - NLM
STAT- MEDLINE
DCOM- 19941122
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 4 Pt 1
DP  - 1994 Oct
TI  - 11 beta-hydroxysteroid dehydrogenase activity in human lung cells and 
      transcription regulation by glucocorticoids.
PG  - L464-74
AB  - Selectivity to aldosterone (Aldo) in mineralocorticoid target tissues has been 
      suggested to be due to the activity of 11 beta-hydroxysteroid dehydrogenase (11 
      beta-HSD). This enzyme inactivates the endogenous glucocorticoid cortisol, thus 
      permitting the unhindered access of Aldo to the mineralocorticoid receptor. The 
      11 beta-HSD activity was measured by the conversion of cortisol to cortisone and 
      vice versa. Concomitant treatment of the cells with either cortisone or cortisol 
      in the presence of the glycyrrhetinic acid derivative carbenoxolone (CBX) blocked 
      both activities of 11 beta-HSD. Dexamethasone and Aldo activated the 
      transcription of transiently transfected mouse mammary tumor virus-bacterial 
      chloramphenicol acetyltransferase chimeric gene in LU-19 cells. The transcription 
      activation by cortisol was synergized by concomitant treatment of the 
      transfectants with CBX. Transactivation with Aldo was inhibited by 
      spironolactone. The enzyme 11 beta-HSD in LU-19 cells is similar to the cloned 
      liver isoform and catalyzes both reduction and dehydrogenation.
FAU - Page, N
AU  - Page N
AD  - Medical Research Council Group in Molecular Endocrinology, Laval University 
      Medical Center, Quebec, Canada.
FAU - Warriar, N
AU  - Warriar N
FAU - Govindan, M V
AU  - Govindan MV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Affinity Labels)
RN  - 0 (Antibodies)
RN  - 0 (Glucocorticoids)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Steroid)
RN  - 9013-72-3 (Hemocyanins)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - FV4Y0JO2CX (keyhole-limpet hemocyanin)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Adult
MH  - Affinity Labels
MH  - Antibodies/immunology
MH  - Cell Line
MH  - Female
MH  - Glucocorticoids/*pharmacology
MH  - Hemocyanins/immunology
MH  - Humans
MH  - Hydrocortisone/antagonists & inhibitors
MH  - Hydroxysteroid Dehydrogenases/*genetics/immunology/*metabolism
MH  - Lung/cytology/*enzymology
MH  - Oxidoreductases/metabolism
MH  - Receptors, Glucocorticoid/metabolism
MH  - Receptors, Steroid/metabolism
MH  - Transcription, Genetic/*drug effects
MH  - Transcriptional Activation
MH  - Ultraviolet Rays
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1152/ajplung.1994.267.4.L464 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Oct;267(4 Pt 1):L464-74. doi: 10.1152/ajplung.1994.267.4.L464.