PMID- 79435
OWN - NLM
STAT- MEDLINE
DCOM- 19781025
LR  - 20190613
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 152
IP  - 2
DP  - 1978 Aug 25
TI  - Electron microscope autoradiographic evidence for specific transneuronal 
      transport in the mouse accessory olfactory bulb.
PG  - 283-302
AB  - The distribution of radioactive material was examined autoradiographically 8 h 
      after application of [3H] proline to the vomeronasal organ in mice. Labelled 
      material was transported along the axons of the vomeronasal nerves to their 
      terminals in the glomerular layer of the accessory olfactory bulb (AOB). A lesser 
      but consistent amount of radioactivity was found in the external plexiform layer 
      (EPL) of the AOB. Electron microscopic autoradiography was used to determine 
      which of the components of the EPL contained this labelled material. The method 
      of proportional grain counts showed that the highest concentration of silver 
      grains lay over the mitral cell dendrites, which are the elements immediately 
      postsynaptic to the vomeronasal nerve axons. However, a fairly high proportion of 
      grains also lay over the peripheral processes of granule cells. By application of 
      a method of 'crossfire analysis' (which is explained in detail) it was possible 
      to show that the observed grain distribution is best explained by the assumption 
      that the radioactive material is confined to mitral cells, and the labelling over 
      granule cell processes is due to crossfire from these sources. Im one animal at 5 
      days after [3H]proline administration label was found to have extended from 
      mitral cells to granule cells, suggesting that the transsynaptically transported 
      radioactive material, which was confined to the mitral cells at 8 h, may have 
      become further redistributed at longer survivals. In a control experiment, 
      [3H]proline was applied directly to the surface of the AOB. This gave rise to a 
      completely different distribution of radioactivity in the EPL: radioactive 
      material was present in all tissue components.
FAU - Barber, P C
AU  - Barber PC
FAU - Parry, D M
AU  - Parry DM
FAU - Field, P M
AU  - Field PM
FAU - Raisman, G
AU  - Raisman G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 10028-17-8 (Tritium)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Animals
MH  - *Autoradiography
MH  - *Axonal Transport
MH  - Axons/ultrastructure
MH  - Central Nervous System/*metabolism
MH  - Cytoplasmic Granules/ultrastructure
MH  - Mice
MH  - Microscopy, Electron
MH  - Nasal Septum/*innervation
MH  - Neuroglia/ultrastructure
MH  - Olfactory Bulb/anatomy & histology/*metabolism
MH  - Olfactory Pathways/anatomy & histology/*metabolism
MH  - Proline/metabolism
MH  - Tritium/metabolism
EDAT- 1978/08/25 00:00
MHDA- 1978/08/25 00:01
CRDT- 1978/08/25 00:00
PHST- 1978/08/25 00:00 [pubmed]
PHST- 1978/08/25 00:01 [medline]
PHST- 1978/08/25 00:00 [entrez]
AID - 0006-8993(78)90256-1 [pii]
AID - 10.1016/0006-8993(78)90256-1 [doi]
PST - ppublish
SO  - Brain Res. 1978 Aug 25;152(2):283-302. doi: 10.1016/0006-8993(78)90256-1.