PMID- 79436
OWN - NLM
STAT- MEDLINE
DCOM- 19781025
LR  - 20190613
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 152
IP  - 2
DP  - 1978 Aug 25
TI  - Inhibition by puromycin of transneuronal transport in the mouse accessory 
      olfactory bulb.
PG  - 303-11
AB  - Application of [3H]proline to the vomeronasal organ (VNO) in mice results in the 
      transport of labelled material along the vomeronasal axons to their terminals in 
      the glomerular layer of the accessory olfactory bulb (AOB). In addition labelled 
      material leaves the vomeronasal nerve terminals and is found over the external 
      plexiform layer (EPL), where a previous electron microscopic autoradiographic 
      study showed that it is preferentially accumulated in mitral cells. Grain 
      densities over the glomerular layer and the EPL were counted in light 
      micrographs. After subtracting background, the overall density of grains in the 
      EPL is about 10% of that over the glomerular layer at 6 h after administration of 
      [3H]proline to the VNO (5 mice). In a further 7 mice, puromycin (or saline) was 
      applied directly to the AOB at hourly intervals during the 6 h after [3H]proline 
      administration. Under these circumstances the labelling in the EPL is only 2--4% 
      of that in the glomerular layer (9% for the 2 saline controls). These 
      observations are evidence that a major part of the transsynaptic transfer 
      mechanism is dependent on protein synthesis, and also favour the view that free 
      amino acids are an important component of the material transferred.
FAU - Barber, P C
AU  - Barber PC
FAU - Raisman, G
AU  - Raisman G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 10028-17-8 (Tritium)
RN  - 4A6ZS6Q2CL (Puromycin)
RN  - 9DLQ4CIU6V (Proline)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Axonal Transport/*drug effects
MH  - Central Nervous System/*metabolism
MH  - Male
MH  - Mice
MH  - Nasal Septum/innervation
MH  - Neurons/metabolism
MH  - Olfactory Bulb/drug effects/*metabolism
MH  - Olfactory Pathways/drug effects/*metabolism
MH  - Proline/metabolism
MH  - Puromycin/*pharmacology
MH  - Tritium/metabolism
EDAT- 1978/08/25 00:00
MHDA- 1978/08/25 00:01
CRDT- 1978/08/25 00:00
PHST- 1978/08/25 00:00 [pubmed]
PHST- 1978/08/25 00:01 [medline]
PHST- 1978/08/25 00:00 [entrez]
AID - 0006-8993(78)90257-3 [pii]
AID - 10.1016/0006-8993(78)90257-3 [doi]
PST - ppublish
SO  - Brain Res. 1978 Aug 25;152(2):303-11. doi: 10.1016/0006-8993(78)90257-3.