PMID- 7943949
OWN - NLM
STAT- MEDLINE
DCOM- 19941109
LR  - 20190914
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 25
IP  - 3
DP  - 1994 Jun
TI  - Integrating the porcine physical and linkage map using cosmid-derived markers.
PG  - 155-64
AB  - An essential part in the development of informative linkage maps is to include 
      genetic markers that have been anchored by physical mapping. Here a set of 18 
      porcine cosmid-derived genetic markers are reported that have been mapped by 
      linkage analysis, and that also have been physically localized by fluorescence in 
      situ hybridization (FISH). Three different strategies were used to establish 
      polymorphic markers from the cosmid clones. Firstly, dinucleotide microsatellite 
      loci were derived by sequencing cosmid subclones containing (CA)n repeats. 
      Secondly, variable SINE 3' poly(A) tracts (SINEVA) were identified by direct 
      SINE-PCR amplification of cosmid clones. Thirdly, the cosmids were used in 
      Southern blot hybridization to detect restriction fragment length polymorphisms 
      (RFLPs). Compared with the most recent consensus compilation of the porcine gene 
      map, the present assignment of markers to chromosomes 2p, 3, 4, 10, 12q, and 16 
      represents the first loci mapped to these chromosomes, for which linkage as well 
      as in situ data are now available.
FAU - Ellegren, H
AU  - Ellegren H
AD  - Department of Animal Breeding and Genetics, Swedish University of Agricultural 
      Sciences, Uppsala.
FAU - Chowdhary, B
AU  - Chowdhary B
FAU - Johansson, M
AU  - Johansson M
FAU - Andersson, L
AU  - Andersson L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Cosmids
MH  - DNA Primers/genetics
MH  - Female
MH  - Genetic Linkage
MH  - Genetic Markers
MH  - Male
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/genetics
MH  - Pedigree
MH  - Polymorphism, Restriction Fragment Length
MH  - Repetitive Sequences, Nucleic Acid
MH  - Swine/*genetics
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1111/j.1365-2052.1994.tb00104.x [doi]
PST - ppublish
SO  - Anim Genet. 1994 Jun;25(3):155-64. doi: 10.1111/j.1365-2052.1994.tb00104.x.