PMID- 7945311
OWN - NLM
STAT- MEDLINE
DCOM- 19941031
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 203
IP  - 3
DP  - 1994 Sep 30
TI  - N omega-hydroxyl-L-arginine, an intermediate in the L-arginine to nitric oxide 
      pathway, is a strong inhibitor of liver and macrophage arginase.
PG  - 1614-21
AB  - N omega-Hydroxy-L-arginine (L-NOHA) is a potent inhibitor of the hydrolysis of 
      L-arginine (L-Arg) to L-ornithine (L-Orn) catalyzed by purified bovine liver 
      arginase (BLA). It appears as one of the most powerful arginase inhibitors 
      reported so far (Ki = 150 microM). The other products of NO synthase are either 
      without effect (NO2-, NO3-) or much weaker inhibitors (L-citrulline (L-Cit) and 
      NO) of BLA. Products derived from a possible hydrolysis of L-Arg (L-Orn and urea) 
      or of L-NOHA (L-Cit, hydroxyurea and hydroxylamine) are also inactive toward BLA 
      at concentrations up to 2 mM. The configuration of L-NOHA is important as D-NOHA 
      is much less active, and its free -COOH and alpha-NH2 functions are required for 
      recognition of BLA. L-NOHA is also a potent inhibitor of the arginase activity of 
      rat liver homogenates and of murine macrophages (IC50 of 150 and 450 microM, 
      respectively). These remarkable properties of L-NOHA could play a role in the 
      modulation of the biosynthesis of the biological mediator NO by increasing local 
      L-Arg concentrations.
FAU - Boucher, J L
AU  - Boucher JL
AD  - Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Universite 
      Paris V, CNRS URA 400, France.
FAU - Custot, J
AU  - Custot J
FAU - Vadon, S
AU  - Vadon S
FAU - Delaforge, M
AU  - Delaforge M
FAU - Lepoivre, M
AU  - Lepoivre M
FAU - Tenu, J P
AU  - Tenu JP
FAU - Yapo, A
AU  - Yapo A
FAU - Mansuy, D
AU  - Mansuy D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 53054-07-2 (N(omega)-hydroxyarginine)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 3.5.3.1 (Arginase)
RN  - HG18B9YRS7 (Valine)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animals
MH  - Arginase/*antagonists & inhibitors
MH  - Arginine/*analogs & derivatives/*metabolism/pharmacology
MH  - Cattle
MH  - Kinetics
MH  - Liver/*enzymology
MH  - Lysine/pharmacology
MH  - Macrophages/*enzymology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Nitric Oxide/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Valine/pharmacology
EDAT- 1994/09/30 00:00
MHDA- 1994/09/30 00:01
CRDT- 1994/09/30 00:00
PHST- 1994/09/30 00:00 [pubmed]
PHST- 1994/09/30 00:01 [medline]
PHST- 1994/09/30 00:00 [entrez]
AID - S0006291X84723710 [pii]
AID - 10.1006/bbrc.1994.2371 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Sep 30;203(3):1614-21. doi: 
      10.1006/bbrc.1994.2371.