PMID- 7945333
OWN - NLM
STAT- MEDLINE
DCOM- 19941031
LR  - 20211203
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 203
IP  - 3
DP  - 1994 Sep 30
TI  - Role of serine/threonine protein kinases in the induction of JE, a 
      platelet-derived growth factor inducible gene.
PG  - 1815-20
AB  - Platelet-derived growth factor (PDGF) and serum both stimulate the transcription 
      of the mouse early response gene, JE. JE and its human homolog, macrophage 
      chemotactic protein-1 (MCP-1), encode potent monocyte chemotactic factors. 
      JE/MCP-1 is a member of the chemokine superfamily of small inducible genes whose 
      products are multifaceted mediators of inflammatory and immune responses. We now 
      report evidence that the serine/threonine kinase inhibitors H7 and H8 but not 
      HA1004, W-7, and ML-7 inhibit the transcriptional induction of the JE gene by 
      serum whereas the phosphatase inhibitor, okadaic acid, increases JE expression. 
      Downregulation of protein kinase C by prior exposure to TPA does not inhibit the 
      induction of JE by serum, nor does it affect the inhibition of JE induction by 
      H7. These results suggest that one or more serine/threonine kinases may be 
      important in the signal transduction mechanism that leads to the induction of the 
      JE gene.
FAU - Kawahara, R S
AU  - Kawahara RS
AD  - Department of Medicine, Jewish Hospital at Washington University Medical Center, 
      St. Louis, MO 63110.
FAU - Deng, Z W
AU  - Deng ZW
FAU - Denkinger, D J
AU  - Denkinger DJ
FAU - Deuel, T F
AU  - Deuel TF
LA  - eng
GR  - CA49712/CA/NCI NIH HHS/United States
GR  - HL14147/HL/NHLBI NIH HHS/United States
GR  - HL31102/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (Ethers, Cyclic)
RN  - 0 (Isoquinolines)
RN  - 0 (Piperazines)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 1W21G5Q4N2 (Okadaic Acid)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - 84478-11-5 (N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
GS  - JE
GS  - MCP-1
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Nucleus/metabolism
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis
MH  - Culture Media
MH  - Cytokines/*biosynthesis
MH  - Ethers, Cyclic/pharmacology
MH  - Gene Expression/*drug effects
MH  - Humans
MH  - Isoquinolines/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Okadaic Acid
MH  - Piperazines/pharmacology
MH  - Platelet-Derived Growth Factor/*pharmacology
MH  - Protein Kinase Inhibitors
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Transcription, Genetic/drug effects
EDAT- 1994/09/30 00:00
MHDA- 1994/09/30 00:01
CRDT- 1994/09/30 00:00
PHST- 1994/09/30 00:00 [pubmed]
PHST- 1994/09/30 00:01 [medline]
PHST- 1994/09/30 00:00 [entrez]
AID - S0006-291X(84)72398-9 [pii]
AID - 10.1006/bbrc.1994.2398 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Sep 30;203(3):1815-20. doi: 
      10.1006/bbrc.1994.2398.