PMID- 7947255
OWN - NLM
STAT- MEDLINE
DCOM- 19941207
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 87
IP  - 1
DP  - 1994 May
TI  - Trisomy 12 in B-cell chronic lymphocytic leukaemia: assessment of lineage 
      restriction by simultaneous analysis of immunophenotype and genotype in 
      interphase cells by fluorescence in situ hybridization.
PG  - 44-50
AB  - We have studied the lineage restriction of trisomy 12 in six patients with B-cell 
      chronic lymphocytic leukaemia (CLL) by simultaneous analysis of immunophenotype 
      and fluorescence in situ hybridization (FISH) signals in single interphase cells. 
      Fresh uncultured cells from each patient were immunophenotyped by the alkaline 
      phosphatase anti-alkaline phosphatase method (APAAP) using monoclonal or 
      polyclonal antibodies and hybridized with a chromosome 12 specific 
      alpha-satellite DNA probe. In all cases trisomy 12 was restricted to the clonal 
      B-cells, kappa positive or lambda positive, whereas T-cells (CD3 positive) and 
      non clonal B-cells had only two chromosome 12 signals. Within the clonal B-cell 
      population a large proportion of cells were disomic for chromosome 12, whilst 
      trisomic cells ranged from 21% to 37%. The absence of trisomy 12 in T-cells and 
      the mosaicism demonstrated in the clonal B-cells suggests that this abnormality 
      is a secondary event during the leukaemic transformation of CLL and develops in 
      an already established neoplastic B-cell population.
FAU - Garcia-Marco, J
AU  - Garcia-Marco J
AD  - Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital, 
      London.
FAU - Matutes, E
AU  - Matutes E
FAU - Morilla, R
AU  - Morilla R
FAU - Ellis, J
AU  - Ellis J
FAU - Oscier, D
AU  - Oscier D
FAU - Fantes, J
AU  - Fantes J
FAU - Catovsky, D
AU  - Catovsky D
FAU - Price, C M
AU  - Price CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Aged
MH  - *Chromosomes, Human, Pair 12
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Neoplastic Stem Cells/pathology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - *Trisomy
MH  - Tumor Cells, Cultured
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1994.tb04868.x [doi]
PST - ppublish
SO  - Br J Haematol. 1994 May;87(1):44-50. doi: 10.1111/j.1365-2141.1994.tb04868.x.