PMID- 7948196
OWN - NLM
STAT- MEDLINE
DCOM- 19941222
LR  - 20220408
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 6
IP  - 3
DP  - 1994 Jul
TI  - A modified serum neutralization test for the detection of antibody to porcine 
      reproductive and respiratory syndrome virus in swine sera.
PG  - 289-92
AB  - Various conditions were evaluated and modified to improve the sensitivity of the 
      serum neutralization (SN) test for detecting antibody in pigs infected with 
      porcine reproductive and respiratory syndrome virus (PRRSV). Higher SN titers 
      were consistently obtained by the addition of 20% fresh swine serum to the virus 
      diluent and by the use of a permissive cell clone (MARC-145) derived from the 
      MA-104 cell line. Test sera used to assess the SN test were obtained from 2 
      groups of 3-week-old pigs infected intranasally with PRRSV (MN-1b). Using the 
      modified method, SN antibody was first detected 9-11 days postinoculation (PI), 
      with a peak evident at 11-21 days PI. The antibody subsequently declined, and a 
      second peak was observed between 41 and 45 days PI. The first antibody peak was 
      not observed and the SN antibody was only detectable between 32 and 41 days PI 
      when the test was done with 20% heated swine serum or without supplemental swine 
      serum. The SN antibody during 2-3 weeks PI was found to be sensitive to 
      2-mercaptoethanol or anti-swine IgM treatment. The SN antibody titers were high 
      when homologous PRRSV isolate was used in the test but were markedly low for 
      heterologous PRRSV isolates. No difference in antibody titers was observed when 
      homologous and heterologous PRRSV isolates were tested by indirect fluorescent 
      antibody assay. These results indicate that the modified SN method is useful in 
      detecting earlier and higher PRRSV antibody and that it can differentiate among 
      PRRSV isolates.
FAU - Yoon, I J
AU  - Yoon IJ
AD  - Department of Clinical and Population Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul 55108.
FAU - Joo, H S
AU  - Joo HS
FAU - Goyal, S M
AU  - Goyal SM
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/*isolation & purification
MH  - Fluorescent Antibody Technique/veterinary
MH  - Neutralization Tests/methods/*veterinary
MH  - Swine
MH  - Swine Diseases/blood/*immunology
MH  - Syndrome
MH  - Time Factors
MH  - Virus Diseases/blood/immunology/*veterinary
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1177/104063879400600326 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 1994 Jul;6(3):289-92. doi: 10.1177/104063879400600326.