PMID- 7948606
OWN - NLM
STAT- MEDLINE
DCOM- 19941212
LR  - 20190920
IS  - 0891-6934 (Print)
IS  - 0891-6934 (Linking)
VI  - 17
IP  - 3
DP  - 1994
TI  - Analyses of lpr-GVHD by adoptive transfer experiments using MRL/lpr-Thy-1.1 
      congenic mice.
PG  - 217-24
AB  - When MRL/Mp- +/+ (MRL/+) mice are lethally irradiated and then reconstituted with 
      MRL/Mp-lpr/lpr (MRL/lpr) spleen and/or bone marrow cells (BMCs), the mice develop 
      a graft-versus-host disease (GVHD)-like syndrome which is known as lpr-GVHD. We 
      analyzed lpr-GVHD by adoptive transfer experiments using congenic MRL/lpr-Thy-1.1 
      mice to distinguish the donor and recipient cells. MRL/+ mice were lethally (9.5 
      Gy) irradiated and then reconstituted with BMCs of MRL/lpr-Thy-1.1 mice treated 
      with anti-Thy-1.1 monoclonal antibody (mAb) plus complement (C). The mice were 
      sacrificed 5 to 6 weeks after bone marrow transplantation (BMT), and the spleen 
      cells were transferred to second recipients. The second recipients (MRL/+ or 
      MRL/lpr mice) were non-irradiated, sublethally (6 Gy) irradiated or lethally (9.5 
      Gy) irradiated. The lethally irradiated mice were also injected with syngeneic 
      BMCs treated with anti-Thy-1.2 mAb plus C. When whole spleen cells (1 x 10(8) 
      were injected into lethally irradiated MRL/+ mice, the mice showed short survival 
      (1.2-1.5 months) and severe histological changes in the spleen (atrophy and 
      fibrosis), liver (lymphoid infiltration in the Glisson's sheath) and lung 
      (lymphoid infiltration around the bronchus and vessel). The sublethally 
      irradiated MRL/+ mice at 2 months after transfer showed histological changes 
      similar to the lethally irradiated MRL/+ recipients, although the former survived 
      more than 3 months, suggesting that histological changes do not reflect on 
      mortality. These GVH-like diseases were not transferable to MRL/lpr mice; they 
      developed autoimmune diseases.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Hosaka, N
AU  - Hosaka N
AD  - First Department of Pathology, Kansai Medical University, Osaka, Japan.
FAU - Nagata, N
AU  - Nagata N
FAU - Nakagawa, T
AU  - Nakagawa T
FAU - Miyashima, S
AU  - Miyashima S
FAU - Yasumizu, R
AU  - Yasumizu R
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Thy-1 Antigens)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/immunology
MH  - Female
MH  - Flow Cytometry
MH  - Graft vs Host Disease/*immunology/pathology
MH  - Immunotherapy, Adoptive/adverse effects
MH  - Lymphocyte Transfusion/*adverse effects
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Mutant Strains
MH  - Spleen/cytology
MH  - T-Lymphocyte Subsets/immunology
MH  - Thy-1 Antigens/immunology
MH  - Whole-Body Irradiation
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.3109/08916939409010657 [doi]
PST - ppublish
SO  - Autoimmunity. 1994;17(3):217-24. doi: 10.3109/08916939409010657.