PMID- 7949008
OWN - NLM
STAT- MEDLINE
DCOM- 19941208
LR  - 20141120
IS  - 1210-0668 (Print)
IS  - 1210-0668 (Linking)
VI  - 28
IP  - 1
DP  - 1994 Mar
TI  - Effect of the in vivo treatment with the synthetic steroid 17 
      beta-methoxy-17-methyl-(5 alpha)-1 H'-androstane-(3,2-c) pyrazole (17MM) on the 
      in vitro formation of DHT in the epididymis of the rat.
PG  - 17-22
AB  - Adult male Sprague-Dawley rats were injected subcutaneously (s.c.) for 21 days 
      either with the synthetic steroid 17 beta-methoxy-17-methyl-(5 alpha)-1 
      H'-androstane-(3,2-c) pyrazole (17MM) at two dose levels (0.1 and 1 mg/kg/day) or 
      with the vehicle used to dilute the steroid. At autopsy, caput epididymes and 
      ventral prostates were removed and incubated in vitro. The effect on the 5 
      alpha-reductase (5 alpha-R) activity was evaluated by measuring the amounts of 
      DHT formed from 14C-testosterone (14C-T) used as the substrate. The in vivo 
      administration of 17MM at the dose of 0.1 mg/kg/day resulted in a significant 
      decrease in the formation of DHT at the level of the epididymis, while there was 
      no effect on the prostate. The dose of 1 mg/kg/day did not induce any 
      modification in the formation of DHT in either organ. The determination of serum 
      LH showed that the treatment with either dose did not alter serum LH titers. 
      These findings suggest that the in vivo treatment with 17MM lowers the in vitro 
      formation of DHT from T at the epididymal, but not at the prostatic level and 
      consequently suggests a selective inhibitory effect of 17MM on the 5 alpha-R 
      present in the epididymis.
FAU - Zoppi, S
AU  - Zoppi S
AD  - Department of Endocrinology, University of Milano, Italy.
FAU - Lechuga, M
AU  - Lechuga M
FAU - Motta, M
AU  - Motta M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Endocr Regul
JT  - Endocrine regulations
JID - 9112018
RN  - 0 (5-alpha Reductase Inhibitors)
RN  - 0 (Androstanes)
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - 5-alpha Reductase Inhibitors
MH  - Androstanes/administration & dosage/*pharmacology
MH  - Animals
MH  - Dihydrotestosterone/*metabolism
MH  - Epididymis/*drug effects/*metabolism
MH  - In Vitro Techniques
MH  - Injections, Subcutaneous
MH  - Luteinizing Hormone/blood
MH  - Male
MH  - Organ Specificity
MH  - Prostate/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PST - ppublish
SO  - Endocr Regul. 1994 Mar;28(1):17-22.