PMID- 7949128
OWN - NLM
STAT- MEDLINE
DCOM- 19941229
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 84
IP  - 11
DP  - 1994 Dec 1
TI  - Platelet adhesion to fibronectin in flow: dependence on surface concentration and 
      shear rate, role of platelet membrane glycoproteins GP IIb/IIIa and VLA-5, and 
      inhibition by heparin.
PG  - 3724-33
AB  - Platelet adhesion to purified surface-immobilized fibronectin under flow 
      conditions was investigated. Fibronectin was found to support attachment and 
      spreading of platelets. The extent of platelet spreading depended on the amount 
      of immobilized fibronectin. An antiglycoprotein (anti-GP) IIb/IIIa antibody and 
      an Arg-Gly-Asp (RGD)-containing peptide inhibited adhesion almost completely, 
      whereas antibodies directed against platelet GP Ic/IIa (very late antigen 5) 
      inhibited by 50%. Similar results with the antibodies and the peptide were found 
      in a static system. A comparison of different anticoagulants showed no difference 
      in adhesion using citrate or hirudin. However, unfractionated heparin (UFH) or 
      low-molecular-weight heparin (LMWH) as the only anticoagulant or in combination 
      with citrate maximally inhibited adhesion by 80% and 60%, respectively. 
      Preincubation of the immobilized fibronectin with UFH resulted in a maximal 
      inhibition of 90%, whereas preincubation with LMWH had no effect. When we 
      preincubated the surface with heparins of different size, we observed 40% 
      inhibition of adhesion with heparins with an average MW of up to 18 kD, whereas a 
      heparin with an average MW of 21 kD almost completely blocked adhesion. These 
      results indicate that platelet adhesion to fibronectin in flow involves several 
      receptors, is highly RGD-mediated, does not require physiologic levels of 
      divalent cations, and can be inhibited by direct binding of heparin to the 
      fibronectin surface.
FAU - Beumer, S
AU  - Beumer S
AD  - Department of Haematology, University Hospital, Utrecht, The Netherlands.
FAU - IJsseldijk, M J
AU  - IJsseldijk MJ
FAU - de Groot, P G
AU  - de Groot PG
FAU - Sixma, J J
AU  - Sixma JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Citrates)
RN  - 0 (Fibronectins)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Hirudins)
RN  - 0 (Oligopeptides)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Receptors, Fibronectin)
RN  - 144161-76-2 (arginyl-glycyl-aspartyl-tryptophan)
RN  - 2968PHW8QP (Citric Acid)
RN  - 9005-49-6 (Heparin)
RN  - DCR9Z582X0 (Epoprostenol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Citrates/pharmacology
MH  - Citric Acid
MH  - Endothelium, Vascular/cytology
MH  - Epoprostenol/immunology/physiology
MH  - Extracellular Matrix/metabolism
MH  - Fibronectins/*metabolism
MH  - Glass
MH  - Heparin/*pharmacology
MH  - Heparin, Low-Molecular-Weight/pharmacology
MH  - Hirudins/pharmacology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligopeptides/pharmacology
MH  - *Platelet Adhesiveness
MH  - Platelet Membrane Glycoproteins/immunology/*physiology
MH  - Receptors, Fibronectin/immunology/*physiology
MH  - Stress, Mechanical
MH  - Surface Properties
MH  - Thrombasthenia/blood
MH  - Umbilical Veins
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - S0006-4971(20)70479-9 [pii]
PST - ppublish
SO  - Blood. 1994 Dec 1;84(11):3724-33.