PMID- 7950361
OWN - NLM
STAT- MEDLINE
DCOM- 19941208
LR  - 20191023
IS  - 0960-8931 (Print)
IS  - 0960-8931 (Linking)
VI  - 4
IP  - 4
DP  - 1994 Aug
TI  - Three-way and two-way rearrangements involving chromosomes 10, 2, 5 and 5, 2 in 
      two marker chromosomes of a human melanoma cell line.
PG  - 259-65
AB  - Two marker chromosomes (mar1 and mar2), provided with two closely spaced 
      heterochromatic bands, were observed in the 14932 cell line established from a 
      human metastatic melanoma. Fluorescence in situ hybridization (FISH) with the 
      alphoid sequence p82H common to all human centromeres showed strong signals over 
      the double C-bands of mar1 and mar2. These were recognized by a chromosome 
      2-specific alphoid probe, although chromosome in situ suppression (CISS) 
      hybridization with a chromosome 2 library failed to reveal any painting along 
      mar1 and mar2. The centromere of mar1 was identified by a chromosome 10-specific 
      alphoid sequence and the marker chromosome was decorated from pter to a region 
      proximal to the interpolated C-band by a chromosome 10 library. The centromere of 
      mar2 could not be recognized by any chromosome-specific alphoid probe, but the 
      whole mar2 was decorated by a chromosome 5 library. This library also painted the 
      distal q arm of mar1, which was not painted by the chromosome 10 library, as well 
      as a small band proximal to the double C-band. Identification of the two marker 
      chromosomes reveals their common origin and indicates a role for chromosomes 2, 5 
      and 10 in the genesis and/or progression of the 14932 melanoma. Alteration to the 
      chromosome-specific alphoid sequence in the centromere of mar2 provides evidence 
      for rearrangement of constitutive heterochromatin alphoid sequences in human 
      tumours.
FAU - Doneda, L
AU  - Doneda L
AD  - Department of Biology and Genetics, Medical Faculty, University of Milan, Italy.
FAU - Wiegant, J
AU  - Wiegant J
FAU - Larizza, L
AU  - Larizza L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Melanoma Res
JT  - Melanoma research
JID - 9109623
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Centromere
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 10
MH  - *Chromosomes, Human, Pair 2
MH  - *Chromosomes, Human, Pair 5
MH  - *Gene Rearrangement
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Melanoma/*genetics/pathology
MH  - Metaphase
MH  - Tumor Cells, Cultured
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1097/00008390-199408000-00008 [doi]
PST - ppublish
SO  - Melanoma Res. 1994 Aug;4(4):259-65. doi: 10.1097/00008390-199408000-00008.