PMID- 7952331 OWN - NLM STAT- MEDLINE DCOM- 19941222 LR - 20190914 IS - 0962-1083 (Print) IS - 0962-1083 (Linking) VI - 3 IP - 5 DP - 1994 Oct TI - Molecular separation of genera in Cassiinae (Leguminosae), and analysis of variation in the nodulating species of Chamaecrista. PG - 507-15 AB - Randomly Amplified Polymorphic DNA (RAPD) methods have been adapted for use as a phenetic tool on the legume tribe Cassiinae. RAPD-generated polymorphism within local populations was lower than between populations from different geographic regions, between species and genera. Examination of three Cassia species, 12 Chamaecrista species and 13 Senna species using eight primers showed the potential for separation of the nodulated/nitrogen fixing genus Chamaecrista from the previously congeneric groups Cassia and Senna. Similarly, RAPD analysis of two groups of nine Ch. rotundifolia and nine Ch. mimosoides samples using 11 primers has given separation according to both species and to geographical location. Analysis of a small sample of five Chamaecrista species from Brazil with eight primers gave separation consistent with known variations in nodule structure. FAU - Whitty, P W AU - Whitty PW AD - Department of Biological Sciences, University of Dundee, UK. FAU - Powell, W AU - Powell W FAU - Sprent, J I AU - Sprent JI LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Mol Ecol JT - Molecular ecology JID - 9214478 SB - IM MH - Base Sequence MH - Cassia/*genetics MH - Fabaceae/*genetics MH - Genetic Variation/*genetics MH - Geography MH - Molecular Sequence Data MH - Nitrogen Fixation MH - Phylogeny MH - *Plants, Medicinal MH - Polymerase Chain Reaction/methods MH - *Polymorphism, Genetic MH - Reproducibility of Results MH - Species Specificity EDAT- 1994/10/01 00:00 MHDA- 1994/10/01 00:01 CRDT- 1994/10/01 00:00 PHST- 1994/10/01 00:00 [pubmed] PHST- 1994/10/01 00:01 [medline] PHST- 1994/10/01 00:00 [entrez] AID - 10.1111/j.1365-294x.1994.tb00129.x [doi] PST - ppublish SO - Mol Ecol. 1994 Oct;3(5):507-15. doi: 10.1111/j.1365-294x.1994.tb00129.x.