PMID- 7954328
OWN - NLM
STAT- MEDLINE
DCOM- 19941207
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 77
IP  - 2
DP  - 1994 Oct 15
TI  - Fluorescence in situ hybridization analysis using cosmid probes to define 
      chromosome 6q abnormalities in ovarian carcinoma cell lines.
PG  - 99-105
AB  - Deletion of 6q is a frequent finding in ovarian carcinoma, which would suggest 
      that this region contains one or more putative tumor suppressor genes. Chromosome 
      6q abnormalities in six ovarian carcinoma cell lines were analyzed by G-banding 
      and fluorescence in situ hybridization (FISH). Using a variety of probes, 
      including a chromosome 6 paint, a probe specific for the chromosome 6 centromere, 
      and cosmids that map to q24 (cCI6-115), q25 (cCI6-4), q26 (cCI6-91, cCI6-119), 
      and q27 (cCI6-13, cCI6-24, and cCI6-111), abnormalities of 6q were found in three 
      cell lines. In cell line OAW42 (hypotetraploid), the sequences complementary to 
      cCI6-119, cCI6-91, and cCI6-13 probes were lost in two homologues of chromosome 
      6, which indicates the deletion of genetic material from bands q26-27. The same 
      bands were translocated in cell line PEO1 (hypertriploid). The probes from this 
      region were absent on two copies of chromosome 6, but hybridized to two or three 
      markers. In cell line 59M (hyperdiploid) an interstitial deletion proximal to q24 
      was detected in one chromosome 6. We conclude that it is very likely that a gene 
      or genes localized in bands 6q26-27, and perhaps in the region proximal to 6q24, 
      play a critical role in the development or progression of ovarian carcinoma.
FAU - Lastowska, M A
AU  - Lastowska MA
AD  - ICRF Department of Medical Oncology, St. Bartholomew's Hospital, West Smithfield, 
      London.
FAU - Lillington, D M
AU  - Lillington DM
FAU - Shelling, A N
AU  - Shelling AN
FAU - Cooke, I
AU  - Cooke I
FAU - Gibbons, B
AU  - Gibbons B
FAU - Young, B D
AU  - Young BD
FAU - Ganesan, T S
AU  - Ganesan TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 6
MH  - Cosmids/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Ovarian Neoplasms/*genetics
MH  - Ploidies
MH  - Tumor Cells, Cultured
EDAT- 1994/10/15 00:00
MHDA- 1994/10/15 00:01
CRDT- 1994/10/15 00:00
PHST- 1994/10/15 00:00 [pubmed]
PHST- 1994/10/15 00:01 [medline]
PHST- 1994/10/15 00:00 [entrez]
AID - 0165-4608(94)90222-4 [pii]
AID - 10.1016/0165-4608(94)90222-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Oct 15;77(2):99-105. doi: 
      10.1016/0165-4608(94)90222-4.