PMID- 7954361
OWN - NLM
STAT- MEDLINE
DCOM- 19941213
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 86
IP  - 1
DP  - 1994 Oct 28
TI  - Asparagine markedly induces the expression of ornithine decarboxylase gene in 
      transformed mammalian cells but not in their untransformed counterparts.
PG  - 97-103
AB  - We have previously shown that asparagine alone induces a 10-15-fold increase in 
      ornithine decarboxylase (ODC) mRNA level in DF-40 mouse neuroblastoma cells. The 
      induction is due to an accumulation of ODC mRNA through a post-transcriptional 
      stabilization mechanism (Chen, Z.P. and Chen, K.Y. (1992) J. Biol. Chem., 267, 
      6946-6951). In the present study we showed that asparagine induced ODC gene 
      expression in v-Ha-ras-transformed 3T3 (ras-3T3) cells but not in 3T3 cells. 
      Other growth related genes including c-src, c-ras, and c-fos were not affected by 
      asparagine in ras-3T3 cells. Southern blot analysis indicated that the pronounced 
      asparagine effect was not due to ODC gene amplification in ras-3T3 cells. The 
      effect of asparagine on the induction of ODC mRNA could account for the 
      significant increases in the ODC activity in ras-3T3 cells. We also examined the 
      effect of asparagine on ODC gene expression in human KD cells and their 
      transformed counterparts. Our findings strongly suggest that the induction of ODC 
      mRNA by asparagine may represent a component of an altered growth regulatory 
      program associated most prominently with cell transformation.
FAU - Chen, Z P
AU  - Chen ZP
AD  - Department of Chemistry, Rutgers University, Piscataway, NJ 08855-0939.
FAU - Chen, K Y
AU  - Chen KY
LA  - eng
GR  - R01 CA49695/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (RNA, Messenger)
RN  - 7006-34-0 (Asparagine)
RN  - EC 4.1.1.17 (Ornithine Decarboxylase)
SB  - IM
GS  - ODC
GS  - c-erbB
GS  - c-fos
GS  - c-myc
GS  - c-ras
GS  - c-src
GS  - ras
GS  - v-Ha-ras
MH  - 3T3 Cells/drug effects/metabolism/physiology
MH  - Animals
MH  - Asparagine/*pharmacology
MH  - Blotting, Southern
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Enzyme Induction
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Genes, ras
MH  - Humans
MH  - Mice
MH  - Oncogenes/drug effects
MH  - Ornithine Decarboxylase/*biosynthesis/*genetics
MH  - Phenotype
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 1994/10/28 00:00
MHDA- 1994/10/28 00:01
CRDT- 1994/10/28 00:00
PHST- 1994/10/28 00:00 [pubmed]
PHST- 1994/10/28 00:01 [medline]
PHST- 1994/10/28 00:00 [entrez]
AID - 0304-3835(94)90185-6 [pii]
AID - 10.1016/0304-3835(94)90185-6 [doi]
PST - ppublish
SO  - Cancer Lett. 1994 Oct 28;86(1):97-103. doi: 10.1016/0304-3835(94)90185-6.