PMID- 7957121
OWN - NLM
STAT- MEDLINE
DCOM- 19941212
LR  - 20191023
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 24
IP  - 3
DP  - 1994
TI  - Localization of the xanthine guanine phosphoribosyl transferase gene (gpt) of E. 
      coli in AS52 metaphase cells by fluorescence in situ hybridization.
PG  - 176-80
AB  - The purpose of this study was to localize the xanthine guanine phosphoribosyl 
      transferase gene (gpt) to a specific chromosome to investigate its proposed 
      autosomal location in the AS52 cell line. AS52 cells are hgprt-deficient Chinese 
      hamster ovary (CHO) cells which carry a single functional copy of the E. coli gpt 
      gene. Fluorescence in situ hybridization (FISH) and digoxigenin-labeled probes, 
      as small as 673 bp, were used in an attempt to localize the 456 bp gpt gene to a 
      specific chromosome. Chi-square analysis of 13 metaphases showed significant 
      labeling on autosomal chromosomes 6 or 7, which are indistinguishable without 
      further banding analysis. Furthermore, a majority of the signals were on the q 
      arm, proximal to the centromere. The data collected supports incorporation of the 
      gpt gene into an acrocentric autosome of the AS52 cell line.
FAU - Michaelis, K C
AU  - Michaelis KC
AD  - Lilly Research Laboratories, A Division of Eli Lilly and Company, Greenfield, 
      Indiana 46140.
FAU - Helvering, L M
AU  - Helvering LM
FAU - Kindig, D E
AU  - Kindig DE
FAU - Garriott, M L
AU  - Garriott ML
FAU - Richardson, K K
AU  - Richardson KK
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - EC 2.4.2.- (Pentosyltransferases)
RN  - EC 2.4.2.22 (xanthine phosphoribosyltransferase)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
GS  - gpt
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells/cytology/metabolism
MH  - Chromosomes, Human, Pair 6/genetics/metabolism/ultrastructure
MH  - Chromosomes, Human, Pair 7/genetics/metabolism/ultrastructure
MH  - Clone Cells
MH  - Cricetinae
MH  - DNA Primers/chemistry
MH  - DNA Probes
MH  - Escherichia coli/*enzymology/genetics
MH  - Fluorescein-5-isothiocyanate
MH  - Gene Expression Regulation, Bacterial/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metaphase/*genetics
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Pentosyltransferases/*genetics
MH  - Plasmids
MH  - Polymerase Chain Reaction
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/em.2850240306 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 1994;24(3):176-80. doi: 10.1002/em.2850240306.