PMID- 7958970
OWN - NLM
STAT- MEDLINE
DCOM- 19941201
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 148
IP  - 2
DP  - 1994 Oct 21
TI  - Cloning and expression of the gene encoding the porcine NADPH oxidase light-chain 
      subunit (p22-phox).
PG  - 363-7
AB  - In previous studies, we showed that interleukin-4 (IL-4) suppressed porcine (p) 
      macrophage superoxide production and that the mechanism of suppression involved 
      down-regulation of the superoxide-generating enzyme NADPH oxidase heavy-chain 
      91-kDa subunit mRNA (gp91-phox) expression. In order to examine the effect of 
      IL-4 on expression of the gene encoding the porcine NADPH oxidase light-chain 
      22-kDa subunit (p22-phox), we cloned the p22-phox cDNA from a macrophage library. 
      The p22-phox cDNA is 786 bp in length and contains a 576-bp open reading frame 
      which predicts a primary translation product of 192 amino acids (aa). Comparison 
      of the porcine and human 22-phox cDNAs showed a high degree of similarity between 
      the two species in their nucleotide (85%) and deduced aa (83%) sequences. as well 
      as in their hydropathy profiles. Notable features, including a high proline 
      content and an iron-coordinating His94, are conserved in both the porcine and 
      human 22-Phox. A single species of mRNA of about 1 kb was detected in 
      macrophages. The mRNA levels remained unchanged in cells treated with 
      lipopolysaccharide (LPS) or with IL-4 at various concentrations from 0-50 ng/ml. 
      Prolonged treatment with LPS or IL-4 did not enhance the effect of these 
      substances on p22-phox mRNA expression. The effect of IL-4 on p22-phox mRNA 
      expression was also compared with another immunosuppressive cytokine, 
      transforming growth factor-beta 1 (TGF beta 1). No change in mRNA expression was 
      found in the cells with or without TGF beta 1 treatment. The results indicated 
      that the heavy and light chains of NADPH oxidase are independently regulated by 
      IL-4 in macrophages.
FAU - Zhou, Y
AU  - Zhou Y
AD  - Department of Veterinary PathoBiology, College of Veterinary Medicine, University 
      of Minnesota, St. Paul 55108.
FAU - Murtaugh, M P
AU  - Murtaugh MP
LA  - eng
SI  - GENBANK/U02476
SI  - GENBANK/U02477
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.1 (CYBA protein, human)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Gene Expression Regulation, Enzymologic
MH  - Interleukin-4/pharmacology
MH  - Kinetics
MH  - Macrophages/metabolism
MH  - *Membrane Transport Proteins
MH  - Molecular Sequence Data
MH  - NADPH Dehydrogenase/*genetics
MH  - NADPH Oxidases
MH  - Phosphoproteins/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Swine
MH  - Transforming Growth Factor beta/pharmacology
EDAT- 1994/10/21 00:00
MHDA- 1994/10/21 00:01
CRDT- 1994/10/21 00:00
PHST- 1994/10/21 00:00 [pubmed]
PHST- 1994/10/21 00:01 [medline]
PHST- 1994/10/21 00:00 [entrez]
AID - 0378-1119(94)90714-5 [pii]
AID - 10.1016/0378-1119(94)90714-5 [doi]
PST - ppublish
SO  - Gene. 1994 Oct 21;148(2):363-7. doi: 10.1016/0378-1119(94)90714-5.