PMID- 7959687
OWN - NLM
STAT- MEDLINE
DCOM- 19941215
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 94
IP  - 5
DP  - 1994 Nov
TI  - Fluorescence in situ hybridization on paraffin-embedded abortion material as a 
      means of retrospective chromosome analysis.
PG  - 518-22
AB  - A fluorescence in situ hybridization (FISH) procedure was used to detect 
      chromosome abnormalities in archival abortion material. Nuclei were isolated from 
      50-microns-thick tissue blocks from 18 selected and karyotyped abortions. Five 
      probes for repetitive centromeric sequences of chromosomes 1, 16, 18, X and Y 
      were used. For each chromosome, at least 200 nuclei were scored blindly, i.e. 
      without knowledge of the karyotype. The FISH results obtained were compatible 
      with the cytogenetic data in 14 cases. There were four discrepancies. Two of 
      these were observed for cases karyotyped as trisomy 16. Furthermore, FISH results 
      showed trisomy 18 in two cases having normal chromosomes 18 and 18q+, 
      respectively. The latter case was not discrepant if the structural rearrangement 
      involved chromosome 18 material. The remaining discrepancies could be explained 
      by chromosomal mosaicism. Admixture of normal maternal cells was also noted. It 
      is concluded that FISH can be used to study retrospectively the presence of 
      chromosome abnormalities in abortion material. However, the quality obtained 
      after the use of fresh material is superior.
FAU - van Lijnschoten, G
AU  - van Lijnschoten G
AD  - Department of Molecular Cell Biology and Genetics, University of Limburg, 
      Maastricht, The Netherlands.
FAU - Albrechts, J
AU  - Albrechts J
FAU - Vallinga, M
AU  - Vallinga M
FAU - Hopman, A H
AU  - Hopman AH
FAU - Arends, J W
AU  - Arends JW
FAU - Geraedts, J
AU  - Geraedts J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
SB  - IM
MH  - Abortion, Spontaneous/*genetics
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Female
MH  - Fetus
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Mosaicism
MH  - Paraffin Embedding
MH  - Pregnancy
MH  - Retrospective Studies
MH  - *Trisomy
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1007/BF00211018 [doi]
PST - ppublish
SO  - Hum Genet. 1994 Nov;94(5):518-22. doi: 10.1007/BF00211018.