PMID- 7959900
OWN - NLM
STAT- MEDLINE
DCOM- 19941212
LR  - 20190825
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 41
IP  - 1
DP  - 1994 Jun
TI  - Characterization of a cytotoxic factor produced by rat bladder cancer cell line: 
      its identity to TNF alpha.
PG  - 19-24
AB  - Clone C19 derived from a rat bladder cancer cell line BC31ad constitutively 
      released cytotoxic factor RCF. The serum-free C19-conditioned medium was 
      partially purified by DEAE-Sephacel, Sephacryl S-200 and MonoQ-FPLC 
      chromatographies to the specific activity of 6.4 x 10(6) U/mg protein (LM assay). 
      RCF was a 17-kDa protein with pI 4.6-5.0. Anti-RCF serum neutralized the 
      cytotoxic activity of rat and mouse, but not human, tumor necrosis factor-alpha 
      (TNF alpha) as well as RCF. The partial amino acid sequence of RCF was homologous 
      with the sequence 43-58 of rat TNF alpha. RCF and rat TNF alpha showed a similar 
      cytotoxic spectrum against several tumor cells. Furthermore, they induced a 
      marked proliferation of mouse T cells in combination with a suboptimal dose of 
      ConA or anti-CD3 antibody, however, human TNF alpha almost not. Taken together, 
      RCF was substantially regarded as rat TNF alpha.
FAU - Hojo, H
AU  - Hojo H
AD  - Department of Hygienic Chemistry, Tohoku University, Sendai, Japan.
FAU - Kaneko, A
AU  - Kaneko A
FAU - Nanya, K
AU  - Nanya K
FAU - Kayagaki, N
AU  - Kayagaki N
FAU - Hashimoto, Y
AU  - Hashimoto Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Killer Factors, Yeast)
RN  - 0 (Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Cytotoxicity, Immunologic
MH  - Killer Factors, Yeast
MH  - Lymphocyte Activation/drug effects
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - *Protein Biosynthesis
MH  - Proteins/chemistry/immunology/isolation & purification
MH  - Rats
MH  - Rats, Inbred ACI
MH  - T-Lymphocytes/immunology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*chemistry/immunology
MH  - Urinary Bladder Neoplasms/*metabolism
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 0165-2478(94)90051-5 [pii]
AID - 10.1016/0165-2478(94)90051-5 [doi]
PST - ppublish
SO  - Immunol Lett. 1994 Jun;41(1):19-24. doi: 10.1016/0165-2478(94)90051-5.