PMID- 7960988
OWN - NLM
STAT- MEDLINE
DCOM- 19941215
LR  - 20190708
IS  - 0360-3016 (Print)
IS  - 0360-3016 (Linking)
VI  - 30
IP  - 4
DP  - 1994 Nov 15
TI  - Use of fluorescence in situ hybridization (FISH) to study chromosomal damage 
      induced by radiation and bromodeoxyuridine in human colon cancer cells.
PG  - 861-6
AB  - PURPOSE: Although the thymidine analog radiation sensitizer bromodeoxyuridine 
      (BrdUrd) increases radiation-induced chromosomal aberrations, it is not known 
      whether these aberrations are uniformly distributed among chromosomes. Using 
      fluorescence in situ hybridization, we carried out a study to test the hypothesis 
      that BrdUrd-induced radiosensitization may be mediated by nonuniform chromosomal 
      damage. METHODS AND MATERIALS: Log phase HT29 human colon cancer cells were 
      exposed to 10 microM BrdUrd (or media alone) for one cell cycle, and the G1 cells 
      were separated by centrifugal elutriation. Half of the control and BrdUrd samples 
      were irradiated with 8 Gy. Cells were then incubated for 24-28 h, and metaphase 
      spreads were prepared. Fluorescence in situ hybridization was performed using 
      paint probes for chromosomes 1 and 4. RESULTS: We found that radiation induced 
      0.20 aberrations per chromosome in chromosome 4. Based on the ratio of the 
      relative lengths of chromosome 1-4 (1.34), it was predicted that chromosome 1 
      would have approximately 0.26 aberrations per chromosome. However, we observed 
      0.39 aberrations per chromosome 1, which was significantly greater than the 
      predicted (p < 0.001 by chi-square). Incubation with BrdUrd prior to irradiation 
      significantly increased the aberrations found in chromosome 1 (by a factor of 
      1.4) and chromosome 4 (by a factor of 1.9) compared to radiation alone (p < 
      0.001) for both chromosome 1 and 4). CONCLUSION: This study demonstrates that 
      individual chromosomes in human colon cancer cells show significantly different 
      rates of aberration after irradiation. Furthermore, the BrdUrd-mediated increase 
      in radiation-induced chromosomal aberrations may not be uniform among 
      chromosomes.
FAU - Wilt, S R
AU  - Wilt SR
AD  - Department of Radiation Oncology, School of Medicine, University of Michigan 
      Medical Center, Ann Arbor 48109-0582.
FAU - Burgess, A C
AU  - Burgess AC
FAU - Normolle, D P
AU  - Normolle DP
FAU - Trent, J M
AU  - Trent JM
FAU - Lawrence, T S
AU  - Lawrence TS
LA  - eng
GR  - CA 42761/CA/NCI NIH HHS/United States
GR  - CA 46592/CA/NCI NIH HHS/United States
GR  - CA 53440/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Int J Radiat Oncol Biol Phys
JT  - International journal of radiation oncology, biology, physics
JID - 7603616
RN  - 0 (Radiation-Sensitizing Agents)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Bromodeoxyuridine/*pharmacology
MH  - Cell Cycle/drug effects/physiology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 1/*drug effects/*radiation effects
MH  - Chromosomes, Human, Pair 4/*drug effects/*radiation effects
MH  - Colonic Neoplasms/drug therapy/*genetics/*radiotherapy
MH  - G1 Phase/drug effects/physiology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Radiation Injuries/etiology
MH  - Radiation Tolerance/*drug effects/*physiology
MH  - Radiation-Sensitizing Agents/*pharmacology
MH  - Tumor Cells, Cultured/drug effects/radiation effects
EDAT- 1994/11/15 00:00
MHDA- 1994/11/15 00:01
CRDT- 1994/11/15 00:00
PHST- 1994/11/15 00:00 [pubmed]
PHST- 1994/11/15 00:01 [medline]
PHST- 1994/11/15 00:00 [entrez]
AID - 0360-3016(94)90361-1 [pii]
AID - 10.1016/0360-3016(94)90361-1 [doi]
PST - ppublish
SO  - Int J Radiat Oncol Biol Phys. 1994 Nov 15;30(4):861-6. doi: 
      10.1016/0360-3016(94)90361-1.