PMID- 7961021
OWN - NLM
STAT- MEDLINE
DCOM- 19941223
LR  - 20190708
IS  - 0360-3016 (Print)
IS  - 0360-3016 (Linking)
VI  - 30
IP  - 5
DP  - 1994 Dec 1
TI  - Prediction of human cell radiosensitivity: comparison of clonogenic assay with 
      chromosome aberrations scored using premature chromosome condensation with 
      fluorescence in situ hybridization.
PG  - 1127-32
AB  - PURPOSE: The purpose of the present investigation was to determine whether 
      chromosome aberrations scored by premature chromosome condensation (PCC) and 
      fluorescence in situ hybridization (FISH) can predict the radiosensitivity of 
      human cell lines, thereby providing a possible means of assessing the in situ 
      radiosensitivity of normal tissues and the radiocurability of individual human 
      cancers. METHODS AND MATERIALS: We used four cells lines of different 
      radiosensitivity: normal human fibroblasts (AG1522), ataxia-telangiectasia 
      fibroblasts (AT2052), a human fibrosarcoma cell line (HT1080), and a human 
      melanoma cell line (melanoma 903). These were irradiated in plateau phase with a 
      range of doses and assessed both for clonogenic cell survival and for aberrations 
      in a single chromosome (number 4) immediately after, and 24 h after irradiation. 
      RESULTS: The initial number of breaks in chromosome 4 was proportional to 
      irradiation dose and was identical for all the different human cell lines, 
      irrespective of radiosensitivity. On the other hand, the number of chromosome 4 
      breaks remaining 24 h after irradiation reflected the radiosensitivity of the 
      cells such that the relationship between residual chromosome aberrations and cell 
      survival was the same for the different cell lines. CONCLUSIONS: These results 
      suggest that the scoring of chromosome aberrations in interphase using FISH with 
      PCC holds considerable promise for predicting the radiosensitivity of normal and 
      tumor tissues in situ.
FAU - Sasai, K
AU  - Sasai K
AD  - Department of Radiation Oncology, Stanford University School of Medicine, CA 
      94305-5468.
FAU - Evans, J W
AU  - Evans JW
FAU - Kovacs, M S
AU  - Kovacs MS
FAU - Brown, J M
AU  - Brown JM
LA  - eng
GR  - R01 CA 55801/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Int J Radiat Oncol Biol Phys
JT  - International journal of radiation oncology, biology, physics
JID - 7603616
SB  - IM
MH  - Ataxia Telangiectasia
MH  - Cell Line
MH  - Cell Survival/radiation effects
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*radiation effects
MH  - Chromosomes, Human, Pair 4
MH  - Colony-Forming Units Assay
MH  - Dose-Response Relationship, Radiation
MH  - Fibroblasts/radiation effects
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Tumor Cells, Cultured
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 0360-3016(94)90319-0 [pii]
AID - 10.1016/0360-3016(94)90319-0 [doi]
PST - ppublish
SO  - Int J Radiat Oncol Biol Phys. 1994 Dec 1;30(5):1127-32. doi: 
      10.1016/0360-3016(94)90319-0.