PMID- 7962115
OWN - NLM
STAT- MEDLINE
DCOM- 19941228
LR  - 20061115
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 161
IP  - 2
DP  - 1994 Nov
TI  - Differential effects of transforming growth factor-beta 1 on distinct 
      developmental stages of murine megakaryocytopoiesis.
PG  - 312-8
AB  - The effect of transforming growth factor-beta 1 (TGF beta 1) on three 
      developmental stages of megakaryocytopoiesis was investigated. Using a murine 
      bone marrow agar culture system, titrated doses of TGF beta 1 were added to 
      cultures assaying primitive high proliferative megakaryocyte progenitors, 
      committed megakaryocyte precursors, and nondividing, endoreduplicating 
      megakaryocytes. The growth of high proliferative megakaryocyte colony-forming 
      cells (HPP-CFU-Mk) that require the growth factors interleukins-1, 3 and 6 (IL-1 
      + IL-3 + IL-6) for colony detection was abrogated by the addition of 1 ng TGF 
      beta 1/ml. The sensitivity of committed megakaryocyte progenitors (colony-forming 
      unit-megakaryocyte, CFU-Mk) to TGF beta 1 depended on the growth factor 
      combination. TGF beta 1 (1 ng/ml) completely inhibited megakaryocyte colony 
      formation from CFU-Mk only in cultures stimulated by low doses of IL-3. TGF beta 
      1 (> 10 ng/ml) could only marginally inhibit megakaryocyte colony formation 
      generated in the presence of either high doses of IL-3 or the combination of low 
      dose IL-3 + IL-6. TGF beta 1 inhibited both IL-3-dependent and IL-6-dependent 
      megakaryocyte growth but tenfold higher doses of TGF beta 1 were required to 
      inhibit growth generated by the combination of IL-3 + IL-6. The data showed that 
      the capacity of TGF beta 1 to inhibit distinct differentiation stages of the 
      megakaryocytopoietic lineage depended on the concentration and combination of 
      growth factors involved.
FAU - Jackson, H
AU  - Jackson H
AD  - Department of Physiology, University of Melbourne, Parkville, Victoria, 
      Australia.
FAU - Williams, N
AU  - Williams N
FAU - Westcott, K R
AU  - Westcott KR
FAU - Green, R
AU  - Green R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Hematopoiesis/*drug effects
MH  - Megakaryocytes/*drug effects/physiology
MH  - Mice
MH  - Stem Cells/cytology/drug effects
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1002/jcp.1041610216 [doi]
PST - ppublish
SO  - J Cell Physiol. 1994 Nov;161(2):312-8. doi: 10.1002/jcp.1041610216.