PMID- 7963269
OWN - NLM
STAT- MEDLINE
DCOM- 19941221
LR  - 20190909
IS  - 0165-1838 (Print)
IS  - 0165-1838 (Linking)
VI  - 49
IP  - 1
DP  - 1994 Sep
TI  - Patch-clamp recordings from subpopulations of autonomic and afferent neurons 
      identified by axonal tracing techniques.
PG  - 85-92
AB  - This study determined whether axonal tracing methods can be used in combination 
      with patch-clamp techniques to examine the electrical properties of identified 
      populations of autonomic and afferent neurons in the adult rat. Fluorescent dyes 
      (Fast Blue, FB and Fluoro-Gold, FG) were injected into the wall of the urinary 
      bladder or colon and into various somatic structures to label postganglionic 
      neurons in the major pelvic ganglia (MPG) as well as visceral and somatic 
      afferent neurons in the lumbosacral dorsal root ganglia (DRG) and trigeminal 
      ganglia (TG). One to 3 weeks after dye injection, neurons were isolated from 
      ganglia by enzymatic dissociation. Following dissociation, single neurons 
      labelled with FB were identified in the three types of ganglion preparations; 
      however FG was only identified consistently in TG neurons. FB was retained in 
      neurons during short-term culture (1-5 days). Following 10 to 20 s exposure to UV 
      light which was required for identification of the cells, whole-cell patch-clamp 
      recordings revealed that the electrophysiological properties of FB-labelled cells 
      did not differ from those of unlabelled cells. However, a more prolonged exposure 
      (1-5 min) of the neurons to UV light produced irreversible damage to the cells 
      which was evident as changes in the action potential, sodium current and resting 
      membrane potential. These results indicate that patch-clamp recording in 
      combination with axonal tracing is a useful approach for studying the electrical 
      properties of identified populations of autonomic and afferent neurons.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Yoshimura, N
AU  - Yoshimura N
AD  - Department of Pharmacology, School of Medicine, University of Pittsburgh, PA 
      15261.
FAU - White, G
AU  - White G
FAU - Weight, F F
AU  - Weight FF
FAU - de Groat, W C
AU  - de Groat WC
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Auton Nerv Syst
JT  - Journal of the autonomic nervous system
JID - 8003419
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Sodium Channels)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Autonomic Nervous System/cytology/*physiology/radiation effects
MH  - Axons/*physiology/radiation effects
MH  - Electrophysiology
MH  - Fluorescent Dyes
MH  - Ganglia, Autonomic/cytology/physiology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Neurons, Afferent/*physiology/radiation effects
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sodium Channels/physiology
MH  - Ultraviolet Rays
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 0165-1838(94)90024-8 [pii]
AID - 10.1016/0165-1838(94)90024-8 [doi]
PST - ppublish
SO  - J Auton Nerv Syst. 1994 Sep;49(1):85-92. doi: 10.1016/0165-1838(94)90024-8.