PMID- 7964121
OWN - NLM
STAT- MEDLINE
DCOM- 19941221
LR  - 20061115
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 124
IP  - 5
DP  - 1994 Nov
TI  - Investigations into the cellular immune responses in latex allergy.
PG  - 638-43
AB  - Latex allergy is a hypersensitivity response to allergens in the natural latex 
      products. Skin prick tests, which do not correlate very well with latex-specific 
      immunoglobulin E (IgE), are yet the only reliable diagnostic tools for clinically 
      determining hypersensitivity to latex. In this study, patients who were skin test 
      (SPT) positive with (STP+/ANA+) or without (SPT+/ANA-) history of anaphylaxis 
      formed the test groups. Healthy volunteers (SPT-/ANA-) served as controls. 
      Lymphocyte proliferation in response to crude and purified latex antigens, serum 
      immunoglobulin E-total, and latex-specific and in vitro immunoglobulin E (IgE) 
      production in response to crude latex antigens were studied. Results indicate 
      that both patient groups showed enhanced response to latex antigen by 
      lymphoproliferation. In patient groups this response to the different antigens 
      tested, or to crude versus purified antigens, was not statistically different. 
      Serum total IgE (ng/ml) was elevated in patients (range, SPT+/ANA-, 40 to 1250; 
      SPT+/ANA+, 40 to 4550) when compared with controls (40 to 450). Low levels (1 to 
      3 ng/ml) of IgE were detected in the peripheral blood mononuclear cell culture 
      supernatants from the patient groups. These levels were < or = 1 ng/ml in the 
      control group. Significant levels of IgE (log titer, mean +/- SD) specific to 
      MFL1 and glove antigens were measured in serum from both SPT+/ANA- (MFL1, 1.85 
      +/- 0.23, p < 0.001; glove, 1.81 +/- 0.23, p < 0.001) and SPT+/ANA- (MFL1, 1.92 
      +/- 0.39, p < 0.007; glove 1.85 +/- 0.4, p < 0.006) and compared with results 
      from the control group (MFL1, 1.50 +/- 0.1; glove, 1.38 +/- 0.19).(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Murali, P S
AU  - Murali PS
AD  - Department of Medicine, Medical College of Wisconsin, Milwaukee.
FAU - Kelly, K J
AU  - Kelly KJ
FAU - Fink, J N
AU  - Fink JN
FAU - Kurup, V P
AU  - Kurup VP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antigens)
RN  - 0 (Latex)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibody Formation
MH  - Antigens/immunology
MH  - Child
MH  - Child, Preschool
MH  - Drug Hypersensitivity/*immunology
MH  - Female
MH  - Humans
MH  - *Immunity, Cellular
MH  - Immunoglobulin E/analysis/biosynthesis/immunology
MH  - *Latex
MH  - Lymphocyte Activation
MH  - Male
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 0022-2143(94)90109-0 [pii]
PST - ppublish
SO  - J Lab Clin Med. 1994 Nov;124(5):638-43.