PMID- 7964167
OWN - NLM
STAT- MEDLINE
DCOM- 19941212
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 56
IP  - 5
DP  - 1994 Nov
TI  - IgE and eosinophil regulation in a murine model of allergic aspergillosis.
PG  - 593-8
AB  - Exposure of BALB/c mice to Aspergillus fumigatus (Af), the antigen responsible 
      for causing allergic bronchopulmonary aspergillosis in humans, caused elevated 
      levels of serum immunoglobulin E (IgE) and peripheral blood and lung eosinophilia 
      similar to that observed in the human disease. We have investigated the role of 
      interleukin-4 (IL-4), IL-5 and interferon-gamma in regulating IgE and 
      eosinophilia in the mouse model. Animals were immunized by intraperitoneal 
      injections of soluble Af antigens adsorbed to alum. These animals developed 
      elevated IgE and Af specific IgG1 and were then treated with anticytokine 
      monoclonal antibodies before the final exposure to particulate Af antigens by the 
      intranasal route. The results showed that anti-IL-5 abrogated eosinophilia in 
      mice, while those treated with anti-IL-4 retained the same or reduced IgE levels 
      compared to pretreatment levels. All anti-IL-5, anti-IFN-gamma, and control 
      antibody-treated animals showed enhanced IgE levels. Anti-IFN-gamma treatment of 
      mice resulted in marked enhancement of eosinophilia compared to all other groups. 
      Eosinophil numbers observed in the histological sections of the lungs confirmed 
      the eosinophilia detected in the peripheral blood. These results indicate that 
      the increase in IgE and eosinophils after exposure to Af antigens in BALB/c mice 
      are due to Af-induced production of IL-4 and IL-5 and that both IgE and 
      eosinophilia are independently regulated.
FAU - Kurup, V P
AU  - Kurup VP
AD  - Department of Medicine, Medical College of Wisconsin, Milwaukee.
FAU - Choi, H
AU  - Choi H
FAU - Murali, P S
AU  - Murali PS
FAU - Coffman, R L
AU  - Coffman RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-5)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/*blood
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antigens, Fungal/administration & dosage/immunology
MH  - Aspergillosis, Allergic Bronchopulmonary/blood/*immunology
MH  - Aspergillus fumigatus/*immunology
MH  - Eosinophilia/blood/*immunology
MH  - Female
MH  - Immunoglobulin E/*blood
MH  - Immunoglobulin G/blood
MH  - Interferon-gamma/antagonists & inhibitors/*physiology
MH  - Interleukin-4/antagonists & inhibitors/*physiology
MH  - Interleukin-5/antagonists & inhibitors/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1002/jlb.56.5.593 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1994 Nov;56(5):593-8. doi: 10.1002/jlb.56.5.593.