PMID- 7964312
OWN - NLM
STAT- MEDLINE
DCOM- 19941227
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 143
IP  - 1
DP  - 1994 Oct
TI  - The effect of ovarian arterial infusion of transforming growth factor alpha on 
      ovarian follicle populations and ovarian hormone secretion in ewes with an 
      autotransplanted ovary.
PG  - 13-24
AB  - Transforming growth factor alpha (TGF alpha) inhibits hormone production by 
      cultured follicular cells but evidence of an effect of TGF alpha on ovarian 
      hormone secretion in vivo is still required. Eleven ewes with an autotransplanted 
      ovary received, by ovarian arterial infusion, either 5 micrograms/h recombinant 
      rat TGF alpha (n = 6) or placebo (n = 5) for 12 h on day 10 of the luteal phase. 
      Two hours before the start and 1 hour before the end of the infusion each ewe 
      received a single injection of gonadotrophin-releasing hormone (GnRH; 150 ng 
      i.v.). Two hours after the end of the infusion luteal regression was induced with 
      prostaglandin F2 alpha (PGF2 alpha; 125 micrograms i.m.). Ovarian and jugular 
      venous blood samples were taken at 10-min, 15-min or 4-h intervals from 2 h 
      before the start of the infusion until 96 h after PGF2 alpha and the rates of 
      secretion of ovarian oestradiol, inhibin, progesterone and androstenedione were 
      determined. Jugular venous concentrations of LH and FSH were also measured and 
      follicle populations monitored by real-time ultrasound scanning. Infusion of TGF 
      alpha resulted in a significant (P < 0.05) depression in the amplitude of the 
      pulsatile response of oestradiol and androstenedione secretion to the 
      GnRH-induced LH pulse at the end of the infusion. Ovarian inhibin secretion was 
      acutely suppressed by TGF alpha infusion (P < 0.001) and remained lower than 
      controls for the period of the experiment. Luteal phase progesterone secretion 
      was also acutely inhibited (P < 0.001) by infusion of TGF alpha, and in one 
      treated ewe progesterone secretion was elevated 48-84 h after PGF2 alpha. Jugular 
      venous concentrations of FSH in TGF alpha-treated ewes were significantly (P < 
      0.001) elevated over controls during the first 48 h of the follicular phase and 
      the LH surge was delayed for about 10 h (P < 0.05). Infusion of TGF alpha caused 
      a marked decline (P < 0.05) in the number of large follicles within 12 h of the 
      end of the infusion. Two of the six treated ewes, including the one with high 
      follicular phase progesterone, had unusually large (8.7 and 10 mm) follicles 
      present from 48-96 h after PGF2 alpha. We conclude that direct arterial infusion 
      of TGF alpha results in acute inhibition of ovarian steroid and inhibin secretion 
      that is associated with induction of atresia in the population of large 
      follicles. The lack of feedback of ovarian hormones results in a rebound increase 
      of FSH which stimulates the growth of more ovarian follicles and the eventual 
      re-establishment of ovarian hormone secretion and normal cyclicity.
FAU - Campbell, B K
AU  - Campbell BK
AD  - CSIRO Division of Animal Production, New South Wales, Australia.
FAU - Gordon, B M
AU  - Gordon BM
FAU - Scaramuzzi, R J
AU  - Scaramuzzi RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 409J2J96VR (Androstenedione)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 57285-09-3 (Inhibins)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Androstenedione/metabolism
MH  - Animals
MH  - Estradiol/metabolism
MH  - Female
MH  - Follicle Stimulating Hormone/blood
MH  - Follicular Atresia/drug effects
MH  - Gonadal Steroid Hormones/*metabolism
MH  - Inhibins/*metabolism
MH  - Luteinizing Hormone/blood
MH  - Ovarian Follicle/drug effects
MH  - Ovary/blood supply/*drug effects/metabolism/transplantation
MH  - Progesterone/metabolism
MH  - Sheep
MH  - Transforming Growth Factor alpha/*pharmacology
MH  - Transplantation, Autologous
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1677/joe.0.1430013 [doi]
PST - ppublish
SO  - J Endocrinol. 1994 Oct;143(1):13-24. doi: 10.1677/joe.0.1430013.