PMID- 7964631 OWN - NLM STAT- MEDLINE DCOM- 19941201 LR - 20191210 IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 75 ( Pt 11) DP - 1994 Nov TI - Nucleotide sequence of the polyhedrin gene region of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus: placement of the virus in lepidopteran nuclear polyhedrosis virus group II. PG - 3211-8 AB - The polyhedrin gene (polh) of Helicoverpa zea single nucleocapsid nuclear polyhedrosis virus (HzSNPV) was identified and shown by sequence analysis of the EcoRI I genomic fragment to encode a 246 amino acid polypeptide that has greater than 80% sequence identity to known polyhedrins. It is preceded by an AT-rich region containing the conserved late promoter motif TAAG, which was identified as a transcription start point. Downstream of polh there were several similarities in genome arrangement to other nuclear polyhedrosis viruses (NPVs). These include open reading frame (ORF) 8, immediately downstream of polh, encoding a 412 amino acid protein with multiple tandem proline residues, which is homologous to ORF8 (ORF1629) of Autographa californica multiple nucleocapsid NPV. Phylogenetic analysis of the polh gene region shows that HzSNPV is a member of the previously described lepidopteran NPV group II and that it is most closely related to polh of the NPVs of Malacosoma nuestria, Spodoptera littoralis, Orgyia pseudotsugata (single nucleocapsid-type virus) and Buzura supressaria. FAU - Cowan, P AU - Cowan P AD - Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia. FAU - Bulach, D AU - Bulach D FAU - Goodge, K AU - Goodge K FAU - Robertson, A AU - Robertson A FAU - Tribe, D E AU - Tribe DE LA - eng SI - GENBANK/U03418 SI - GENBANK/U11242 SI - GENBANK/Z12117 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (Occlusion Body Matrix Proteins) RN - 0 (Viral Core Proteins) RN - 0 (Viral Proteins) RN - 0 (Viral Structural Proteins) RN - 0 (polyhedrin protein, Nucleopolyhedrovirus) SB - IM GS - polh MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Capsid/*genetics MH - Conserved Sequence MH - *Genes, Viral MH - Molecular Sequence Data MH - Moths/virology MH - Nucleopolyhedroviruses/*genetics MH - Occlusion Body Matrix Proteins MH - Open Reading Frames MH - *Phylogeny MH - Promoter Regions, Genetic MH - Sequence Homology, Amino Acid MH - Viral Core Proteins/*genetics MH - Viral Proteins/*genetics MH - Viral Structural Proteins EDAT- 1994/11/01 00:00 MHDA- 1994/11/01 00:01 CRDT- 1994/11/01 00:00 PHST- 1994/11/01 00:00 [pubmed] PHST- 1994/11/01 00:01 [medline] PHST- 1994/11/01 00:00 [entrez] AID - 10.1099/0022-1317-75-11-3211 [doi] PST - ppublish SO - J Gen Virol. 1994 Nov;75 ( Pt 11):3211-8. doi: 10.1099/0022-1317-75-11-3211.