PMID- 7964636
OWN - NLM
STAT- MEDLINE
DCOM- 19941201
LR  - 20131121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 75 ( Pt 11)
DP  - 1994 Nov
TI  - Inhibition of human T cell leukaemia virus type I long terminal repeat expression 
      by DNA methylation: implications for latency.
PG  - 3255-9
AB  - Human T cell leukaemia virus type I (HTLV-I) provirus DNA was found to be 
      methylated in patients with adult T cell leukaemia. We have therefore examined 
      the possibility that DNA methylation might contribute to HTLV-I latency. In vitro 
      methylation of HTLV-I long terminal repeat (LTR)-chloramphenicol 
      acetyltransferase or LTR-Luciferase constructs at eight HpaII sites, a subset of 
      the eukaryotic methylation site CpG, resulted in a three- to fourfold inhibition 
      of transcription in transfected cells. Inhibition of transcription by methylation 
      of all CpG methylation sites using SssI methylase was much more pronounced (50- 
      to 80-fold). As partial methylation of the LTR showed, methylation of the 
      promoter region was responsible for most of the effect. Whereas cellular 
      stimulation by a combination of phorbol 12-myristate 13-acetate and Tax was able 
      to reverse the HpaII methylation effect, the inhibition by SssI methylation was 
      not suppressible under these conditions. The results are in line with a possible 
      function of DNA methylation in HTLV-I latency.
FAU - Cassens, S
AU  - Cassens S
AD  - Abteilung Virologie, Robert Koch-Institut, Berlin, Germany.
FAU - Ulrich, U
AU  - Ulrich U
FAU - Beimling, P
AU  - Beimling P
FAU - Simon, D
AU  - Simon D
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, tax)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.1.1.- (DNA modification methylase HpaII)
RN  - EC 2.1.1.- (DNA-Cytosine Methylases)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Chloramphenicol O-Acetyltransferase/biosynthesis
MH  - DNA, Viral/chemistry/*metabolism
MH  - DNA-Cytosine Methylases/metabolism
MH  - Gene Products, tax/metabolism
MH  - Human T-lymphotropic virus 1/*genetics/isolation & purification/physiology
MH  - Humans
MH  - Leukemia, T-Cell/virology
MH  - Luciferases/biosynthesis
MH  - Methylation
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - *Transcription, Genetic
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1099/0022-1317-75-11-3255 [doi]
PST - ppublish
SO  - J Gen Virol. 1994 Nov;75 ( Pt 11):3255-9. doi: 10.1099/0022-1317-75-11-3255.