PMID- 7967509 OWN - NLM STAT- MEDLINE DCOM- 19941129 LR - 20071114 IS - 0023-6837 (Print) IS - 0023-6837 (Linking) VI - 71 IP - 4 DP - 1994 Oct TI - Glomerular expression of monocyte chemoattractant protein-1 in experimental and human glomerulonephritis. PG - 536-42 AB - BACKGROUND: Glomerular monocytes are found in a variety of renal diseases and appear to be important in the pathogenesis of glomerular injury. The mediators responsible for recruiting monocytes to the glomerulus are not well characterized. We have recently established that cultured human mesangial cells produce the specific monocyte chemoattractant, monocyte chemoattractant protein-1 (MCP-1) in response to inflammatory cytokines commonly found in glomeruli during glomerulonephritis (GN). This suggested that MCP-1 may be involved in recruiting leukocytes to the glomerulus. To date however, there is little information regarding the expression of MCP-1 during renal disease. The present study was undertaken to investigate the in vivo expression of MCP-1 during experimental and human GN. EXPERIMENTAL DESIGN: A rodent model of anti-glomerular basement membrane GN was used to investigate glomerular MCP-1 mRNA regulation and protein expression. The presence of MCP-1 in human renal tissue was studied by immunostaining kidney biopsy material from patients with a variety of glomerulopathies. RESULTS: MCP-1 mRNA was transiently upregulated during the early stages of experimental GN. Message levels increased in association with the monocyte influx and correlated with expression of immunoreactive MCP-1 in the nephritic glomeruli. Glomerular MCP-1 was also found in human inflammatory glomerulopathies, but not in glomerular diseases lacking a prominent monocyte infiltrate. CONCLUSIONS: These data support a role for MCP-1 in the pathogenesis of glomerular inflammation. FAU - Rovin, B H AU - Rovin BH AD - Department of Medicine, Ohio State University School of Medicine, Columbus. FAU - Rumancik, M AU - Rumancik M FAU - Tan, L AU - Tan L FAU - Dickerson, J AU - Dickerson J LA - eng GR - R29 DK46055/DK/NIDDK NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Lab Invest JT - Laboratory investigation; a journal of technical methods and pathology JID - 0376617 RN - 0 (Chemokine CCL2) RN - 0 (Chemotactic Factors) RN - 0 (RNA, Messenger) RN - 9007-49-2 (DNA) SB - IM MH - Animals MH - Blotting, Northern MH - Chemokine CCL2 MH - Chemotactic Factors/*analysis/genetics/metabolism MH - DNA/analysis/genetics MH - Disease Models, Animal MH - Gene Expression Regulation MH - Glomerulonephritis/genetics/*metabolism/pathology MH - Humans MH - Immunohistochemistry MH - Kidney Glomerulus/*chemistry/metabolism/pathology MH - Male MH - RNA, Messenger/analysis/genetics MH - Rats MH - Rats, Inbred Lew EDAT- 1994/10/01 00:00 MHDA- 1994/10/01 00:01 CRDT- 1994/10/01 00:00 PHST- 1994/10/01 00:00 [pubmed] PHST- 1994/10/01 00:01 [medline] PHST- 1994/10/01 00:00 [entrez] PST - ppublish SO - Lab Invest. 1994 Oct;71(4):536-42.