PMID- 7971514
OWN - NLM
STAT- MEDLINE
DCOM- 19941220
LR  - 20190914
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 25
IP  - 5
DP  - 1994 Nov
TI  - Two-phase short-term culture method for cytogenetic investigations from human 
      prostate carcinoma.
PG  - 225-35
AB  - An improved technique for primary short-term culture of prostate carcinoma cells 
      in two phases, with and without serum, for subsequent cytogenetic analysis is 
      reported and compared with four other methods. After mechanical disaggregation 
      and a brief collagenase treatment of tumor specimens, cell clusters were seeded 
      in RPMI 1640 and 15% fetal calf serum (FCS) without any other supplement in the 
      first phase. The culture medium was changed to a serum-free medium supplemented 
      with bovine pituitary extract (BPE) and epidermal growth factor (EGF) when the 
      first outgrowth became apparent. During this second phase, fibroblast growth 
      could be virtually abolished within 48 hr. The epithelial and prostatic origin of 
      the cultured cells was confirmed by immunocytochemical methods in each culture. 
      Metaphase analysis revealed chromosome aberrations in over 80% of cases (both 
      clonal and nonclonal alterations) indicating the presence of neoplastic cells. 
      Clonal numerical chromosome aberrations, found by conventional cytogenetic 
      analysis, were used to provide the reliability of the culture system in 
      interphase nuclei of corresponding uncultured tumor tissue by fluorescence in 
      situ hybridization (FISH). The main points of the described method are: 1) 
      combined mechanical/enzymatic disaggregation, 2) seeding of the disaggregated 
      cell clumps rather than of single cells, 3) initialization of the cultures in 
      RPMI 1640 medium with 18% FCS without any other supplements, and (4) stimulating 
      of selective epithelial proliferation by changing the culture conditions through 
      serum-free medium.
FAU - Szucs, S
AU  - Szucs S
AD  - Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische 
      Universitat Munchen (Klinikum rechts der Isar), Germany.
FAU - Zitzelsberger, H
AU  - Zitzelsberger H
FAU - Breul, J
AU  - Breul J
FAU - Bauchinger, M
AU  - Bauchinger M
FAU - Hofler, H
AU  - Hofler H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Antigens, Neoplasm/analysis
MH  - Cell Division
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - Culture Techniques/methods
MH  - Cytogenetics/methods
MH  - Humans
MH  - Immunohistochemistry
MH  - Karyotyping
MH  - Male
MH  - Mitosis
MH  - Neoplasm Staging
MH  - Prostatic Neoplasms/*genetics/*pathology
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1002/pros.2990250502 [doi]
PST - ppublish
SO  - Prostate. 1994 Nov;25(5):225-35. doi: 10.1002/pros.2990250502.