PMID- 7975453
OWN - NLM
STAT- MEDLINE
DCOM- 19941229
LR  - 20190819
IS  - 0042-9007 (Print)
IS  - 0042-9007 (Linking)
VI  - 67
IP  - 1
DP  - 1994
TI  - Frozen platelet plates for platelet antibody detection and cross-match.
PG  - 52-7
AB  - We performed 2 studies aimed at developing a frozen platelet panel suitable for 
      platelet cross-matching. The stability of the most important platelet membrane 
      glycoproteins and the reactivity of antigens of the human platelet antigen (HPA) 
      and of the human leukocyte antigen (HLA) systems were evaluated with the platelet 
      suspension immunofluorescence test (PSIFT) in a panel of platelets frozen in 
      microplates with 6% dimethylsulfoxide. In study No. 1 we evaluated platelet 
      reaction with a broad-spectrum weak anti-HLA and a potent anti-HPA-1a antiserum 
      and the expression of glycoproteins Ib and IIb/IIIa complex on platelet membrane 
      before freezing and after 0.5, 1, 2, 3, 4, 5, 6 and 12 months of storage at -80 
      degrees C. In study No. 2 we examined platelet reactivity with anti-HPA-1b, 
      -HPA-2a, -HPA-3a, -HLA-A2, -HLA-A3 of platelets stored frozen for 12 months in 
      parallel with fresh platelets from the same donors. Study No. 1 showed that 
      glycoprotein expression was stable and that the weak anti-HLA and the potent 
      anti-HPA-1a antibodies were clearly detected during 12 months at -80 degrees C. 
      Of the 35 paired PSIFT performed in study No. 2 with fresh and frozen/thawed 
      platelets incubated with anti-HPA-1b, -HPA-2a, -HPA-3a, -HLA-A2, -HLA-A3 antisera 
      and AB serum, concordant reactions were obtained in all cases with the exception 
      of 1 case of HLA-A3-positive platelets incubated with anti-HLA-A3 antiserum, that 
      was reactive with frozen/thawed platelets but nonreactive with fresh platelets 
      from the same donor.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Porretti, L
AU  - Porretti L
AD  - Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore, Milano, 
      Italia.
FAU - Marangoni, F
AU  - Marangoni F
FAU - Rebulla, P
AU  - Rebulla P
FAU - Sirchia, G
AU  - Sirchia G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vox Sang
JT  - Vox sanguinis
JID - 0413606
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (Isoantibodies)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Antigens, Human Platelet/*immunology
MH  - Blood Donors
MH  - Blood Platelets/*immunology
MH  - *Cryopreservation
MH  - Fluorescent Antibody Technique
MH  - Histocompatibility Testing/*instrumentation
MH  - Humans
MH  - Immunization
MH  - Isoantibodies/*analysis
MH  - Platelet Membrane Glycoproteins/immunology
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1111/j.1423-0410.1994.tb05038.x [doi]
PST - ppublish
SO  - Vox Sang. 1994;67(1):52-7. doi: 10.1111/j.1423-0410.1994.tb05038.x.