PMID- 7977739
OWN - NLM
STAT- MEDLINE
DCOM- 19941207
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 5 Pt 1
DP  - 1994 Nov
TI  - Bradykinin and FMLP stimulate prostanoid production by adult rabbit colonocytes 
      in culture.
PG  - G778-85
AB  - Normal colonocytes in culture produce prostaglandins both constitutively and in 
      response to inflammatory stimuli. These highly purified proliferative cell 
      populations were isolated from normal adult rabbit proximal and distal colon. 
      Basal prostaglandin production ranged from 3.4 to 11.7 ng.15 min-1 x 10(6) 
      cells-1. Cultures were incubated at 37 degrees C in the presence or absence of 
      bradykinin or N-formyl-methionine-leucine-phenylalanine (FMLP) over 
      concentrations from 10(-9) to 10(-5) M. In both distal and proximal colonocytes 
      bradykinin stimulated a dose-dependent increase in prostaglandin E2 (PGE2) and 
      6-keto-PGF1 alpha, the stable metabolite of prostacyclin; production peaked at 
      10(-6) M. Proximal colonocytes responded to FMLP with a bell-shaped curve, with 
      maximal stimulation of both PGE2 and 6-keto-PGF1 alpha occurring at 10(-8) M. 
      Distal colonocytes responded variably to FMLP. Arachidonic acid also stimulated 
      prostanoid production in a concentration-dependent manner, with maximal 
      stimulation occurring at 100 microM. The full synthetic profile of prostanoid 
      production was determined by labeling with [14C]arachidonic acid and by analyzing 
      metabolites using radiochromatography on reverse-phase high-pressure liquid 
      chromatography. Only PGE2 and 6-keto-PGF1 alpha were detected. A similar profile 
      of labeled metabolites occurred when colonocytes were prelabeled with 
      [14C]arachidonic acid and stimulated with bradykinin or FMLP. The degradative 
      capacity of the colonocytes appeared very low. Colonocyte production of 
      protective prostaglandins in response to luminal or other inflammatory stimuli 
      may serve as a mucosal defense mechanism. Prostanoids so produced may also 
      modulate the functions of colonocytes, surrounding cells, or both.
FAU - LeDuc, L E
AU  - LeDuc LE
AD  - Department of Pharmacology, Milton S. Hershey Medical Center, Pennsylvania State 
      University, Hershey 17033.
FAU - Brown, L
AU  - Brown L
FAU - Vidrich, A
AU  - Vidrich A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Prostaglandins)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Animals
MH  - Bradykinin/*pharmacology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Colon/cytology/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Female
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Prostaglandins/agonists/classification/*metabolism
MH  - Rabbits
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1152/ajpgi.1994.267.5.G778 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Nov;267(5 Pt 1):G778-85. doi: 10.1152/ajpgi.1994.267.5.G778.