PMID- 7977753
OWN - NLM
STAT- MEDLINE
DCOM- 19941207
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 5 Pt 1
DP  - 1994 Nov
TI  - Characterization of a Y1-preferring NPY/PYY receptor in HT-29 cells.
PG  - G901-7
AB  - Equilibrium binding studies showed that butyrate-treated HT-29 cells express a 
      high-affinity 125I-labeled peptide YY (125I-PYY) binding site with a dissociation 
      constant of 0.32 +/- 0.12 nM (mean +/- SE, n = 4). This site was Y1 preferring 
      because neuropeptide Y (NPY) and the Y1-selective agonist [Leu31,Pro34]NPY were 
      equipotent to PYY at displacing 125I-PYY; PYY-(13-36) and pancreatic polypeptide 
      were > 1,000- and 10,000-fold less potent at displacing the radioligand. PYY and 
      [Leu31,Pro34]NPY inhibited forskolin-stimulated adenosine 3',5'-cyclic 
      monophosphate production 63% and 48%, respectively, with a half-maximal 
      inhibitory concentration between 0.1 and 1.0 nM. PYY and [Leu31,Pro34]NPY had no 
      effect on release of intracellular calcium alone or on the increase in 
      intracellular calcium concentration caused by carbachol or neurotensin. Northern 
      blot analysis of poly(A)+ RNA from HT-29 cells demonstrated a single transcript 
      of 2.5 kb that hybridized to a human Y1-receptor cDNA probe. Sequence analysis of 
      a reverse transcription-polymerase chain reaction product amplified with primers 
      based on human Y1-receptor cDNA confirmed that these cells contained mRNA 
      encoding the human Y1 receptor. These studies show that butyrate-treated HT-29 
      cells constitutively express the Y1-preferring NPY/PYY receptor and Y1 mRNA and 
      provide a new model for studies of PYY-regulated epithelial cell function and 
      tissue-specific expression of the human Y1-receptor gene.
FAU - Mannon, P J
AU  - Mannon PJ
AD  - Division of Gastroenterology, Duke University Medical Center, Durham, North 
      Carolina 27710.
FAU - Mervin, S J
AU  - Mervin SJ
FAU - Sheriff-Carter, K D
AU  - Sheriff-Carter KD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Butyrates)
RN  - 0 (Gastrointestinal Hormones)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Gastrointestinal Hormone)
RN  - 0 (Receptors, Neuropeptide Y)
RN  - 0 (peptide YY receptor)
RN  - 106388-42-5 (Peptide YY)
RN  - 107-92-6 (Butyric Acid)
RN  - E0399OZS9N (Cyclic AMP)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Binding Sites
MH  - Blotting, Northern
MH  - Butyrates/pharmacology
MH  - Butyric Acid
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Cyclic AMP/antagonists & inhibitors
MH  - Gastrointestinal Hormones/pharmacology
MH  - Humans
MH  - Intestinal Mucosa/cytology/drug effects/*metabolism
MH  - Intracellular Membranes/metabolism
MH  - Peptide YY
MH  - Peptides/pharmacology
MH  - Polymerase Chain Reaction
MH  - Receptors, Gastrointestinal Hormone/*classification/*metabolism
MH  - Receptors, Neuropeptide Y/*classification/*metabolism
MH  - Transcription, Genetic
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1152/ajpgi.1994.267.5.G901 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Nov;267(5 Pt 1):G901-7. doi: 10.1152/ajpgi.1994.267.5.G901.