PMID- 7979872
OWN - NLM
STAT- MEDLINE
DCOM- 19941202
LR  - 20190904
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 162
IP  - 3
DP  - 1994
TI  - Phosphorylation of glucose by a guanosine-5'-triphosphate (GTP)-dependent 
      glucokinase in Fibrobacter succinogenes subsp. succinogenes S85.
PG  - 180-6
AB  - Cell extracts of Fibrobacter succinogenes subsp. succinogenes S85 phosphorylated 
      glucose with a GTP-dependent glucokinase. The enzyme showed little activity with 
      ATP (12% of that with GTP). Of other phosphate donors tested, only dGTP and ITP 
      gave high glucokinase activities. Dialyzed extracts required Mg+2 and K+ for 
      maximal activity. In potassium phosphate buffer, glucokinase showed maximum 
      activity at pH 7.5 with glucose-6-phosphate dehydrogenase as the coupling enzyme. 
      In this assay, glucokinase was active with glucose (100%), 2-deoxy-D-glucose 
      (40%), and mannose (20%). Partially purified glucokinase had a molecular weight 
      of 82,000 and a pI of 4.82. Double-reciprocal plots of substrate concentration 
      versus velocity were linear and the enzyme had apparent Km values of 55 microM 
      for glucose and 72 microM for GTP. Dialyzed cell extracts of Fibrobacter 
      intestinalis C1A also contained a GTP-dependent glucokinase that showed little 
      activity with ATP. Potassium also stimulated the activity of this enzyme. These 
      results suggest that this unusual glucokinase may be characteristic of the genus 
      Fibrobacter.
FAU - Glass, T L
AU  - Glass TL
AD  - Department of Veterinary and Microbiological Sciences, North Dakota State 
      University, Fargo 58105.
FAU - Sherwood, J S
AU  - Sherwood JS
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Cations)
RN  - 0 (Phosphates)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.7.1.2 (Glucokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cations
MH  - Cattle
MH  - Glucokinase/chemistry/isolation & purification/*metabolism
MH  - Glucose/*metabolism
MH  - Gram-Negative Anaerobic Bacteria/*metabolism
MH  - Guanosine Triphosphate/*metabolism
MH  - Kinetics
MH  - Molecular Weight
MH  - Phosphates/metabolism
MH  - Phosphorylation
MH  - Rumen/microbiology
MH  - Substrate Specificity
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF00314472 [doi]
PST - ppublish
SO  - Arch Microbiol. 1994;162(3):180-6. doi: 10.1007/BF00314472.