PMID- 7980602
OWN - NLM
STAT- MEDLINE
DCOM- 19941222
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 204
IP  - 3
DP  - 1994 Nov 15
TI  - Dopachrome tautomerase is a zinc-containing enzyme.
PG  - 1243-50
AB  - Dopachrome tautomerase (DCT) catalyzes the conversion of L-dopachrome into 
      5,6-dihydroxyindole-2-carboxylic acid through the melanogenic biosynthetic 
      pathway. This enzyme, also named TRP2, belongs to the family of the tyrosinase 
      related proteins. The three members of the family contain two highly conserved 
      metal-binding sites with three histidines on each. Tyrosinase has copper at its 
      active site. It was assumed that although DCT might have copper in those metal 
      binding sites, its active site could be related to other two putative 
      iron-binding sites located in different positions. Based on apoDCT preparation 
      with cyanide and reconstitution experiments, we propose that DCT have zinc 
      instead of copper at the two metal-binding sites and that those sites actually 
      correspond to the active site. The involvement of zinc, which cannot undergo 
      redox reactions, accounts for the reaction that DCT catalyzes, a tautomerization 
      versus the copper-mediated oxidations catalyzed by tyrosinase.
FAU - Solano, F
AU  - Solano F
AD  - Dept. of Biochemistry & Molecular Biology, School of Medicine, University of 
      Murcia, Spain.
FAU - Martinez-Liarte, J H
AU  - Martinez-Liarte JH
FAU - Jimenez-Cervantes, C
AU  - Jimenez-Cervantes C
FAU - Garcia-Borron, J C
AU  - Garcia-Borron JC
FAU - Lozano, J A
AU  - Lozano JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoenzymes)
RN  - 0 (Metalloproteins)
RN  - 789U1901C5 (Copper)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - EC 5.- (Isomerases)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.3.12 (dopachrome isomerase)
RN  - J41CSQ7QDS (Zinc)
RN  - MQD255M2ZO (Potassium Cyanide)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoenzymes/chemistry
MH  - Binding Sites
MH  - Cell Line
MH  - Conserved Sequence
MH  - Copper/analysis
MH  - Edetic Acid/pharmacology
MH  - Enzyme Stability
MH  - *Intramolecular Oxidoreductases
MH  - Isomerases/*chemistry/isolation & purification/metabolism
MH  - Kinetics
MH  - Melanocytes/*enzymology
MH  - Melanoma, Experimental/enzymology
MH  - Metalloproteins/*chemistry/isolation & purification/metabolism
MH  - Mice
MH  - Monophenol Monooxygenase/chemistry/metabolism
MH  - Potassium Cyanide/pharmacology
MH  - Zinc/*analysis
EDAT- 1994/11/15 00:00
MHDA- 1994/11/15 00:01
CRDT- 1994/11/15 00:00
PHST- 1994/11/15 00:00 [pubmed]
PHST- 1994/11/15 00:01 [medline]
PHST- 1994/11/15 00:00 [entrez]
AID - S0006291X84725964 [pii]
AID - 10.1006/bbrc.1994.2596 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Nov 15;204(3):1243-50. doi: 
      10.1006/bbrc.1994.2596.