PMID- 7983082
OWN - NLM
STAT- MEDLINE
DCOM- 19950103
LR  - 20061115
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 28
IP  - 8
DP  - 1994 Aug
TI  - In vitro chondrocyte collagen deposition within porous HDPE: substrate 
      microstructure and wettability effects.
PG  - 839-50
AB  - The effects of microstructure and wettability of porous high density polyethylene 
      (HDPE) substrates on chondrocyte collagen synthesis in vitro were assayed. Three 
      size grades of hydrophilic and hydrophobic HDPE substrates with ranges of pore 
      volumes of 40-60%, pore sizes of 115-335 microns, and surface areas per unit 
      volumes of 7-20 mm2/mm3 were seeded with fetal bovine chondrocytes. After 7 days 
      of incubation, the cells within all substrates remained spherical, and contained 
      mainly type II collagen (as verified by type I and II collagen I-ELISAs). After 
      21 days, the majority of cells had spread; however, the matrices still contained 
      mainly type II collagen. The hydrophilic matrices contained significantly more 
      type II collagen than the hydrophobic matrices at both 7 and 21 days, whereas the 
      amount of type II collagen was not influenced by the pore attributes. A 
      significantly higher percentage of type II collagen was also observed in all 
      seeded porous substrates as compared with seeded polystyrene culture dishes, 
      perhaps indicating that the three-dimensional particular nature of the HDPE 
      matrices enhanced the maintenance of phenotypically differentiated chondrocytes 
      and entrapment of their extracellular matrix products.
FAU - LiVecchi, A B
AU  - LiVecchi AB
AD  - Department of Bioengineering, Clemson University, South Carolina 29634.
FAU - Tombes, R M
AU  - Tombes RM
FAU - LaBerge, M
AU  - LaBerge M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Polyethylenes)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*cytology/*metabolism
MH  - Cattle
MH  - Cells, Cultured
MH  - Collagen/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoblotting
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Fluorescence
MH  - Particle Size
MH  - *Polyethylenes/chemistry
MH  - Porosity
MH  - Surface Properties
MH  - Tissue Embedding
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1002/jbm.820280802 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 1994 Aug;28(8):839-50. doi: 10.1002/jbm.820280802.