PMID- 7984049
OWN - NLM
STAT- MEDLINE
DCOM- 19950105
LR  - 20190825
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 25
IP  - 1-2
DP  - 1994 Aug
TI  - Repeated administration of MDMA down-regulates preprocholecystokinin mRNA 
      expression but not tyrosine hydroxylase mRNA expression in neurones of the rat 
      substantia nigra.
PG  - 34-40
AB  - The effect of repeated administration of 3,4-methylenedioxymethamphetamine (MDMA) 
      on the expression of tyrosine hydroxylase and preprocholecystokinin (CCK) 
      messenger RNAs in substantia nigra was examined by in situ hybridisation 
      histochemistry. Sections hybridised with 35S-labelled oligonucleotides were 
      subjected to computerised image analysis to determine the density of silver 
      grains above positively labelled cells as an index of steady state mRNA levels. 
      In the substantia nigra pars compacta, CCK mRNA levels were significantly reduced 
      in drug-treated animals 24 h and at 2 weeks after the last dose of MDMA (10 mg/kg 
      i.p., twice daily for 4 days). In the same animals, MDMA caused no change in the 
      level of tyrosine hydroxylase mRNA in this brain region. The results show that 
      MDMA can produce changes in dopamine neurones. Furthermore, since tyrosine 
      hydroxylase and cholecystokinin are co-expressed in substantia nigra pars 
      compacta, these results suggest that the expression of the tyrosine hydroxylase 
      and CCK genes are regulated independently.
FAU - Wotherspoon, G
AU  - Wotherspoon G
AD  - Molecular Neuropharmacology Laboratory, UMDS Division of Biochemistry and 
      Molecular Biology, University of London, Guy's Hospital, UK.
FAU - Savery, D
AU  - Savery D
FAU - Priestley, J V
AU  - Priestley JV
FAU - Rattray, M
AU  - Rattray M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Messenger)
RN  - 89624-02-2 (preprocholecystokinin)
RN  - 9011-97-6 (Cholecystokinin)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Cholecystokinin/*genetics
MH  - Down-Regulation/drug effects
MH  - Genetic Code
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Neurons/*drug effects/metabolism
MH  - Protein Precursors/*genetics
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Substantia Nigra/cytology/*drug effects/metabolism
MH  - Tyrosine 3-Monooxygenase/*biosynthesis
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 0169-328X(94)90275-5 [pii]
AID - 10.1016/0169-328x(94)90275-5 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1994 Aug;25(1-2):34-40. doi: 
      10.1016/0169-328x(94)90275-5.