PMID- 7985028
OWN - NLM
STAT- MEDLINE
DCOM- 19950103
LR  - 20190618
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 266
IP  - 5190
DP  - 1994 Dec 2
TI  - Reconstitution of an operational MHC class II compartment in 
      nonantigen-presenting cells.
PG  - 1569-73
AB  - Professional antigen-presenting cells (APCs) have a distinct compartment in which 
      class II molecules are proposed to acquire antigenic peptides. Genetic evidence 
      suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, 
      participates in this process. Peptide acquisition was reconstituted in 
      nonprofessional APCs by transfection of class II, invariant chain (li), and H-2M, 
      the murine equivalent of DM. The H-2M heterodimer appeared in an endosomal 
      compartment, not at the cell surface, and the localization was independent of li. 
      The data presented show that H-2M, class II, and li are the minimally required 
      components for efficient formation of stable class II-peptide complexes, and thus 
      for a functional class II compartment.
FAU - Karlsson, L
AU  - Karlsson L
AD  - R. W. Johnson Pharmaceutical Research Institute, Scripps Research Institute, La 
      Jolla, CA 92037.
FAU - Peleraux, A
AU  - Peleraux A
FAU - Lindstedt, R
AU  - Lindstedt R
FAU - Liljedahl, M
AU  - Liljedahl M
FAU - Peterson, P A
AU  - Peterson PA
LA  - eng
GR  - AI-26610/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (H-2 Antigens)
RN  - 0 (HLA-DR3 Antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (invariant chain)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigen-Presenting Cells/immunology
MH  - *Antigens, Differentiation, B-Lymphocyte
MH  - B-Lymphocytes/immunology
MH  - Cell Line
MH  - Cell Membrane/immunology
MH  - Endosomes/*immunology
MH  - Fluorescent Antibody Technique
MH  - H-2 Antigens/analysis/genetics/*metabolism
MH  - HLA-DR3 Antigen/*metabolism
MH  - HeLa Cells
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Transfection
EDAT- 1994/12/02 00:00
MHDA- 1994/12/02 00:01
CRDT- 1994/12/02 00:00
PHST- 1994/12/02 00:00 [pubmed]
PHST- 1994/12/02 00:01 [medline]
PHST- 1994/12/02 00:00 [entrez]
AID - 10.1126/science.7985028 [doi]
PST - ppublish
SO  - Science. 1994 Dec 2;266(5190):1569-73. doi: 10.1126/science.7985028.