PMID- 7986039
OWN - NLM
STAT- MEDLINE
DCOM- 19950105
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 60
IP  - 10
DP  - 1994 Oct
TI  - Degradation of methyl bromide by methanotrophic bacteria in cell suspensions and 
      soils.
PG  - 3640-6
AB  - Cell suspensions of Methylococcus capsulatus mineralized methyl bromide (MeBr), 
      as evidence by its removal from the gas phase, the quantitative recovery of Br- 
      in the spent medium, and the production of 14CO2 from [14C]MeBr. Methyl fluoride 
      fluoride (MeF) inhibited oxidation of methane as well as that of [14C]MeBr. The 
      rate of MeBr consumption by cells varied inversely with the supply of methane, 
      which suggested a competitive relationship between these two substrates. However, 
      MeBr did not support growth of the methanotroph. In soils exposed to high levels 
      (10,000 ppm) of MeBr, methane oxidation was completely inhibited. At this 
      concentration, MeBr removal rates were equivalent in killed and live controls, 
      which indicated a chemical rather than biological removal reaction. At lower 
      concentration (1,000 ppm) of MeBr, methanotrophs were active and MeBr consumption 
      rates were 10-fold higher in live controls than in killed controls. Soils exposed 
      to trace levels (10 ppm) of MeBr demonstrated complete consumption within 5 h of 
      incubation, while controls inhibited with MeF or incubated without O2 had 50% 
      lower removal rates. Aerobic soils oxidized [14C]MeBr to 14CO2, and MeF inhibited 
      oxidation by 72%. Field experiments demonstrated slightly lower MeBr removal 
      rates in chambers containing MeF than in chambers lacking MeF. Collectively, 
      these results show that soil methanotrophic bacteria, as well as other microbes, 
      can degrade MeBr present in the environment.
FAU - Oremland, R S
AU  - Oremland RS
AD  - U.S. Geological Survey, Menlo Park, California 94025.
FAU - Miller, L G
AU  - Miller LG
FAU - Culbertson, C W
AU  - Culbertson CW
FAU - Connell, T L
AU  - Connell TL
FAU - Jahnke, L
AU  - Jahnke L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Environmental Pollutants)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 9V42E1Z7B6 (methyl bromide)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Environmental Pollutants/metabolism
MH  - Hydrocarbons, Brominated/*metabolism
MH  - Methylococcaceae/*metabolism
MH  - Oxidation-Reduction
MH  - Soil Microbiology
PMC - PMC201867
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
PMCR- 1994/10/01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
PHST- 1994/10/01 00:00 [pmc-release]
AID - 10.1128/aem.60.10.3640-3646.1994 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1994 Oct;60(10):3640-6. doi: 
      10.1128/aem.60.10.3640-3646.1994.