PMID- 7986725
OWN - NLM
STAT- MEDLINE
DCOM- 19950109
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 87
IP  - 4
DP  - 1994 Aug
TI  - Detection of trisomy 12 in chronic lymphocytic leukaemia: comparison of a 
      polymerase chain reaction based technique with fluorescence in situ 
      hybridization.
PG  - 843-5
AB  - Trisomy 12 is the most frequent chromosomal aberration in chronic lymphocytic 
      leukaemia (CLL) and seems to indicate a poor prognosis. To detect this 
      abnormality we tested the applicability of the polymerase chain reaction (PCR) 
      and compared it to the current standard fluorescence in situ hybridization 
      (FISH). Two DNA regions containing variable numbers of tandem repeats (VNTR) 
      located on (a) the long and (b) the short arm of chromosome 12 were chosen for 
      PCR analysis. 8/72 patients (11%) were trisomy 12 positive compared to 16% by 
      FISH. Chromosomal imbalances were only detected by PCR if at least 20% of the 
      cells carried the numerical aberration.
FAU - Reining, G
AU  - Reining G
AD  - Clinical Institute of Medical and Chemical Laboratory Diagnosis, University of 
      Vienna, Austria.
FAU - Clodi, K
AU  - Clodi K
FAU - Konig, M
AU  - Konig M
FAU - Geissler, K
AU  - Geissler K
FAU - Haas, O A
AU  - Haas OA
FAU - Mannhalter, C
AU  - Mannhalter C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - *Chromosomes, Human, Pair 12
MH  - Gene Dosage
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - *Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - *Trisomy
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1111/j.1365-2141.1994.tb06748.x [doi]
PST - ppublish
SO  - Br J Haematol. 1994 Aug;87(4):843-5. doi: 10.1111/j.1365-2141.1994.tb06748.x.