PMID- 7988280 OWN - NLM STAT- MEDLINE DCOM- 19950112 LR - 20150901 IS - 0253-2662 (Print) IS - 0253-2662 (Linking) VI - 26 IP - 4 DP - 1993 Nov TI - [Reductive dechlorination of chlorophenols by an anaerobic mixed culture]. PG - 139-50 AB - In this study, we investigated the potential for reductive dechlorination of chlorophenols by municipal sewage sludge acclimated to 2,4-dichlorophenol (2,4-DCP) and 3,4-dichlorophenol (3,4-DCP). The optimal temperature and pH for dechlorination of 2,4,6-trichlorophenol (2,4,6-TCP) were 30 degrees C and 7.2. Dechlorination of 2,4,6-TCP was inhibited by sulfate, nitrate, or ferric (III), but was increased by manganese (IV) except that product of 2,4-DCP was accumulated. Organic substrates such as pyruvate or acetate did not influence dechlorination of 2,4,6-TCP, but increased dechlorination of 2,4-DCP; nevertheless lactate showed no influence on dechlorination. When glucose was added as substrate, only 20% of 2,4,6-TCP dechlorination was found. Addition of methanogenesis inhibitor 2-bromoethane sulfonate did not influence dechlorination, but addition of eubacteria inhibitor vancomycin showed no dechlorination activity at the first few days, and finally complete transformation and accumulation of 2,4-DCP occurred. Because mixed culture was from Di-Hwa Wastewater Treatment Plant, adding the same water as culture medium was found to increase the dechlorination of 2,4,6-TCP and its product 2,4-DCP. Dechlorination by adding wastewater of Petroleum Corporation was also increased, especially for 2,4-DCP product, but only 7% of 2,4,6-TCP dechlorination was found after incubation with Hou-Chin river water for 14 days. FAU - Chang, B V AU - Chang BV AD - Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China. FAU - Chen, K S AU - Chen KS FAU - Yuan, S Y AU - Yuan SY LA - jpn PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China (Republic : 1949- ) TA - Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi JT - Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology JID - 8008067 RN - 0 (Chlorophenols) SB - IM MH - Bacteria, Anaerobic/*metabolism MH - Chlorophenols/*metabolism MH - Hydrogen-Ion Concentration MH - Oxidation-Reduction MH - Temperature EDAT- 1993/11/01 00:00 MHDA- 1993/11/01 00:01 CRDT- 1993/11/01 00:00 PHST- 1993/11/01 00:00 [pubmed] PHST- 1993/11/01 00:01 [medline] PHST- 1993/11/01 00:00 [entrez] PST - ppublish SO - Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1993 Nov;26(4):139-50.