PMID- 7988424
OWN - NLM
STAT- MEDLINE
DCOM- 19950106
LR  - 20061115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 135
IP  - 6
DP  - 1994 Dec
TI  - Fibroblast growth factor receptor type 1 expression during rat testicular 
      development and its regulation in cultured Sertoli cells.
PG  - 2404-11
AB  - In the present study, we examined the ontogeny of the type 1 receptor for basic 
      fibroblast growth factor (FGFR-1) in whole rat testis, its cellular localization, 
      and its in vitro regulation in 20-day-old rat Sertoli cells. Gene expression of 
      FGFR-1 was developmentally regulated; expression was higher in prepubertal testes 
      and decreased with sexual maturity. The transcript was found to be expressed in 
      Leydig-enriched fractions, peritubular cells, Sertoli cells, and, to a lesser 
      extent, germ cells. FSH as well as (Bu)2cAMP enhanced FGFR-1 messenger RNA (mRNA) 
      levels in cultured Sertoli cells, suggesting an involvement of the protein 
      kinase-A pathway. Addition of basic FGF (bFGF), tumor necrosis factor-alpha (TNF 
      alpha), or interleukin-1 alpha resulted in a dose- and time-related increase in 
      FGFR-1 mRNA levels. The effect of bFGF was specific, because it was neutralized 
      by cotreatment with an anti-bFGF. We tested medium conditioned by germ cells and 
      found a stimulation of the Sertoli cell FGFR-1 mRNA levels, which was abolished 
      by immunodepletion of the conditioned medium with anti-TNF alpha antibodies. It 
      is suggested that in Sertoli cells, bFGF action, when mediated by FGFR-1, is 
      under a complex hormonal (FSH) and paracrine and/or autocrine control exerted at 
      least by bFGF, TNF alpha, and interleukin-1 alpha.
FAU - Le Magueresse-Battistoni, B
AU  - Le Magueresse-Battistoni B
AD  - INSERM U-407, Centre Hospitalier Lyon-Sud, Pierre-Benite, France.
FAU - Wolff, J
AU  - Wolff J
FAU - Morera, A M
AU  - Morera AM
FAU - Benahmed, M
AU  - Benahmed M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Fibroblast Growth Factor)
SB  - IM
MH  - Aging/metabolism
MH  - Animals
MH  - Animals, Newborn/growth & development/metabolism
MH  - Culture Media, Conditioned/metabolism/pharmacology
MH  - Gene Expression
MH  - Germ Cells/metabolism
MH  - Kinetics
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Fibroblast Growth Factor/genetics/*metabolism
MH  - Sertoli Cells/*metabolism
MH  - Testis/cytology/*growth & development/*metabolism
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1210/endo.135.6.7988424 [doi]
PST - ppublish
SO  - Endocrinology. 1994 Dec;135(6):2404-11. doi: 10.1210/endo.135.6.7988424.